MULTISCALE CHARACTERIZATION OF MONOSYNAPTIC CIRCUITS IN THE BRAIN

这个子项目是许多利用 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 一种新的基于狂犬病病毒的方法，用于映射神经元回路中的单突触连接，将用于（1）标记神经元和与这些神经元相关的突触前末梢，以及（2）确定是否所有撞击识别的突触后神经元的突触前末梢都通过这种方法进行了跨突触标记。NCMIR在相关光学显微镜（LM）和电子显微镜（EM）标记化学的发展将扩展到与该系统一起工作，从而能够对保存良好的标本中的标记终端和相关结构进行高分辨率评估。使用正在开发的新方法来生产 你好¿¿图像也将被探索，以使这些方法的分层，以解决有关的问题，详细分布的多个分子成分在确定的突触复合体。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. A new rabies virus-based method for mapping monosynaptic connections in neuronal circuits will be used to (1) label neurons and pre-synaptic terminals associated with those neurons and (2) determine if all pre-synaptic terminals impinging on the identified postsynaptic neuron are trans-synaptically marked by this approach. NCMIR developments in labeling chemistry for correlated light microscopy (LM) and electron microscopy (EM) will be extended to work with this system enabling high-resolution assessment of labeled terminals and associated structures in well-preserved specimens. The use of the new method being developed to produce ¿¿multicolor EM¿¿ images will also be explored to enable layering of these methods to address questions regarding detailed distribution of multiple molecular constituents in identified synaptic complexes.