THE ROLE OF NMDA RECEPTOR ON SYNAPTIC DEVELOPMENT

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 NMDA受体在树突和棘突生长发育中的作用尚未完全确定。由于完整的NR1基因敲除在出生时死亡，因此NR1皮质基因敲除是研究NMDA受体在出生后发育中作用的有价值的工具。我们一直在使用电生理学、共聚焦和电子显微镜(连续切片重建和电子断层扫描)来研究这些小鼠的皮质突触，以了解NMDA受体在脊椎和兴奋性突触发育中的作用。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The role of NMDA receptors on the growth and development of dendrites and spines are not fully established. The NR1 cortical knockout is a valuable tool to study the role of NMDA receptors in postnatal development since the full NR1 knockout dies at birth. We have been studying the cortical synapses in these mice using electrophysiology, confocal and electron microscopy (serial section reconstruction and electron tomography) to understand the role of NMDA receptors on the development of spines and excitatory synapses.