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Analysis of Neural Circuits using a Genetically-encoded Silencer

使用基因编码消音器分析神经回路

基本信息

批准号：
7937912
负责人：
ANIRVAN GHOSH
金额：
$ 38.38万
依托单位：
UNIVERSITY OF CALIFORNIA, SAN DIEGO
依托单位国家：
美国
项目类别：
财政年份：
2009
资助国家：
美国
起止时间：
2009-09-30 至 2011-08-31
项目状态：
已结题

项目摘要

DESCRIPTION (provided by applicant): This application addresses broad Challenge Area (06): Enabling Technologies and specific Challenge Topic, 06-NS-103: Breakthrough technologies for neuroscience. The goal of this project is to develop a strategy to examine the role of specific cell types in the function of cortical circuits. As a proof of principle we have chosen to focus on GABAergic neurons since they can regulate depolarization of postsynaptic excitatory neurons with great spatial and temporal precision, and are likely to play a critical role in regulating the output of cortical activity. We propose to engineer mice in which the activity of GABAergic subpopulations can be acutely and reversibly blocked so that we can assess their contribution to the function of cortical circuits. The proposed technology would be generally useful to the neuroscience community since similar approaches could be used to silence different neuronal populations to study their function in vivo. PUBLIC HEALTH RELEVANCE: The goal of this project is to develop a strategy to examine the role of specific cell types in the function of cortical circuits. As a proof of principle we have chosen to focus on GABAergic neurons since they can regulate depolarization of postsynaptic excitatory neurons with great spatial and temporal precision, and are likely to play a critical role in regulating the output of cortical activity. We propose to engineer mice in which the activity of GABAergic subpopulations can be acutely and reversibly blocked so that we can assess their contribution to the function of cortical circuits.

描述（由申请人提供）：本申请涉及广泛的挑战领域（06）：使能技术和特定的挑战主题，06-NS-103：神经科学的突破性技术。本项目的目标是开发一种策略来检查特定细胞类型在皮层回路功能中的作用。作为原理的证明，我们选择专注于GABA能神经元，因为它们可以以很大的空间和时间精度调节突触后兴奋性神经元的去极化，并且可能在调节皮质活动的输出中发挥关键作用。我们建议设计小鼠，其中GABA能亚群的活性可以被急性和可逆地阻断，这样我们就可以评估它们对皮层回路功能的贡献。所提出的技术通常对神经科学界有用，因为类似的方法可以用于沉默不同的神经元群体以研究它们在体内的功能。公共卫生相关性：本项目的目标是开发一种策略来检查特定细胞类型在皮层回路功能中的作用。作为原理的证明，我们选择专注于GABA能神经元，因为它们可以以很大的空间和时间精度调节突触后兴奋性神经元的去极化，并且可能在调节皮质活动的输出中发挥关键作用。我们建议设计小鼠，其中GABA能亚群的活性可以被急性和可逆地阻断，这样我们就可以评估它们对皮层回路功能的贡献。