Core A Biology and Antibody Core

概述 转基因小鼠模型和抗体试剂的生产使生物医学发生了革命性的变化 研究(1-4)。这些研究工具使我们有可能回答许多无法回答的问题 并帮助确定了特定基因产物在复杂过程中的作用，如 如脂肪生成和肥胖、糖尿病和血浆脂代谢。 本计划项目资助(PPG)的小鼠模型和抗体核心的主要目标是 制造基因靶向的小鼠，并为这三个项目制备单克隆和多克隆抗体。 所有三个项目都将使用转基因小鼠模型和多克隆或单克隆抗体。 产生新的小鼠品系和产生抗体既昂贵又耗时，而且两者都 需要相当多的技术专长。尽管老鼠和抗体试剂对许多人来说是至关重要的 现代生物医学研究项目，大多数实验室很难保持必要的 技术在任何时候都“悬而未决”。通常情况下，当一名技术人员 离开去另一个机构或博士后研究员去教员的职位。这样效率就会很低， 在实验室内重新建立这些技术既昂贵又痛苦。因此，这是有道理的， 特别是对于PPG来说，将这些职能集中在一个核心设施中。在这场PPG中，我们很幸运地 继承BayGenome人员(Fong、Young、Reue、Hu博士)在创建基因目标方面的技术专长 小鼠，以及Bensadoun、Fong和Young博士在生产 单抗和多克隆抗体。

Overview The production of genetically-modified mouse models and antibody reagents has revolutionized biomedical research (1-4). These research tools have made it possible to answer many questions that cannot be addressed otherwise, and have helped to define the roles of specific gene products in complex processes such as adipogenesis and obesity, diabetes mellitus, and plasma lipid metabolism. The principal objectives of the Mouse Model and Antibody Core of this Program Project Grant (PPG) are to generate gene-targeted mice and to prepare both monoclonal and polyclonal antibodies for the three projects. Genetically modified mouse models and polyclonal or monoclonal antibodies will be used in all three projects. It is both expensive and time consuming to generate new lines of mice and to create antibodies, and both require considerable technical expertise. Even though mouse and antibody reagents are critical for many modern biomedical research projects, it is very difficult for the majority of laboratories to keep the requisite techniques "up in the air" at all times. Often, the required technical expertise disappears when a technician leaves for another institution or a postdoctoral fellow departs for a faculty position. It is then inefficient, expensive, and painful to re-establish the techniques within the laboratory. Therefore, it makes sense, particularly for a PPG, to centralize these functions within a core facility. In this PPG, we are fortunate to inherit the technical expertise of BayGenomics personnel (Drs. Fong, Young, Reue, Hu) in creating genetargeted mice, and the substantial experience of Drs. Bensadoun, Fong, and Young in the production of monoclonal and polyclonal antibodies.