DNA MICROARRAY AND EXPRESSION CORE

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 TNPRC的DNA微阵列和表达核心(微阵列核心)目前提供以下服务： A.DNA微阵列： 1)微阵列实验设计： 2)斑点或双色微阵列实验： 3)基因芯片数据分析 4)小样本放大 5)聚合酶链式反应阵列 6)RNA提取 B.基于siRNA的基因沉默： 1)设计和合成对TNPRC社区有用的siRNA的子集 2)个体siRNA检测方法的优化和标准化 2008年，核心向TNPRC的各种调查人员开出了64,684.50美元的账单，其中包括在核心进行的各种分析的人工费用22,195.74美元，不包括数据分析。此外，核心还支持那些将自己的试剂带到我们这里的研究人员，他们进行实验，只收取人工费用。至少有三名调查人员以这种方式使用了核心。 在2008年，CORE处理了大约500个独特的RNA(或cDNA)样本，并为不同的研究人员进行了总共213次独特的微阵列杂交。这导致产生了近2 TB(TB)的DNA微阵列数据(包括原始数据和分析数据)。其中一些数据已经公布，而更多的数据目前正在分析中。 在未来几年，核心将扩展到以下研究服务领域： 1.定制微阵列：芯体将根据需求生产多个定制微阵列。第一批将由CORE设计、制造和生产的阵列将包括恒河猴组织阵列(肠道、大脑)、泛猕猴淋巴芯片和用于监测“无病原体”猕猴群体的多病原体检测芯片。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The DNA Microarray and Expression Core (Microarray Core) at TNPRC currently provides the following services: A. DNA Microarrays: 1) Microarray experimental design: 2) Spotted or 2-color microarray experiments: 3) Microarray Data Analysis 4) Small Sample Amplification 5) PCR Arrays 6) RNA Isolation B. siRNA based gene-silencing: 1) Design and synthesis of a subset of siRNA's useful to the TNPRC community 2) Optimization and standardization of individual siRNA assay In the year 2008, the core billed $ 64,684.50 to various TNPRC investigators including $ 22,195.74 in labor charges for the various assays performed in the core, excluding data analysis. In addition the core supported investigators who brought their own reagents to us, by performing their experiments and charging for only labor. At least three investigators used the core in this manner. During the year 2008, the core handled ~500 unique RNA (or cDNA) samples, and performed 213 total unique microarray hybridizations for the various investigators. This resulted in the generation of close to 2 TB (TERABYTES) of DNA Microarray data (including both raw and analyzed data). Some of this data has already been published, while much more is currently being analyzed. In the coming years, the core would like to expand into the following research service areas: 1. Custom microarrays: The core will produce several custom microarrays based on demand. The first few arrays that will be designed, fabricated and produced by the core will include rhesus macaque tissue arrays (Gut, brain), a pan-macaque lympho-chip and a multi-pathogen detection chip for surveillance of the "pathogen-free" macaque colony.