DIETARY PROTEIN INTAKE IN MAINTENANCE OF HEMODIALYSIS PATIENTS

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 本项目由两项研究组成。研究1包括9至13名维持性血液透析(MHD)患者，他们临床稳定，将接受氮平衡研究，同时每人摄入17天的膳食蛋白质摄入量，提供0.60、0.80、1.00、1.15和1.30克蛋白质/公斤/天。13C-亮氨酸周转研究13C-尿素动力学研究和血浆氨基酸、透析液氨基酸、多肽和蛋白质的测量，肌肉活检以测量IGF-I、IGF-II、IGF受体、肌肉抑制素和IGF结合蛋白的蛋白质和基因表达，以及身体组成人体测量和双X-射线质子吸收法DXA。 在研究2中，70-80名MHD门诊患者将被随机分配到1.00或1.30g/kg/天的DPI，为期5个月。总蛋白质将通过测量总氮和总钾来评估。身体成分的其他成分将使用人体测量、DXA、BIA和近红外电抗进行评估。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. This project is composed of two studies. Study 1 consists of 9 to 13 maintenance hemodialysis (MHD) patients who are clinically stable and will undergo nitrogen balance studies while they ingest, for 17 days each, diet protein intakes providing 0.60, 0.80, 1.00, 1.15 and 1.30 g protein/kg/day. 13 C-leucine turnover studies 13C-urea kinetic studies and measurements of plasma amino acides, dialysate amino acids, peptides and proteins, muscle biopsies for measurement of protein and gene expression for IGF-I, IGF-II, IGF-receptor, myostatin, and IGF-binding proteins and body composition anthropometry and dual x-ray pohton absorptiometry DXA will be measured. In Study 2, 70-80 MHD outpatients will be randomly assigned to a DPI of 1.00 or 1.30 g/kg/day for five months each. Total body protein will be assessed by measurements of total body nitrogen and total body potassium. Other components of body composition will be assessed using anthropometry, DXA, BIA and near infrared interactance.