USE OF F2 BEAMLINE TO COLLECT MAD DATA FOR OUTER MEMBRANE PROTEIN PAPC

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 PapC是一种位于革兰氏阴性菌外膜的引座子，负责病原性P菌毛的组装和分泌。它也是所谓的“供体-链交换”发生的平台。PapC的晶体结构将为理解引导菌毛亚单位/伴侣复合体的整合、菌毛亚单位的移位以及菌毛的组装提供重要信息-所谓的伴侣-引毛生物发生途径的基本机制。我们已经结晶了PAPC，在家用X光机上，晶体的衍射角达到了3.2埃。晶体属I422，晶胞尺寸a=b=120，c=350。我们需要波束时间来收集MAD或SAD数据集，以确定第一个引座者结构。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. PapC is an usher located in the outer membrane of Gram-negative bacteria, responsible for the assembly and secretion of pathogenic P pilus. It is also a platform for the so-called `donor-strand exchange` taking place. Crystal strucutre of PapC will provide important information for understanding how usher recongizing the pilus subunit/chaperone complex, how to translocate pilus subunits, and how to assemle the pilus --- the basic mechanism of so called chaperone-usher pilus biogenesis pathway. We have crystallized PapC, and the crystals diffracted to ~3.2 angstrom at home x-ray machine. the crystals belong to I422, with cell dimentions: a=b=120, c=350. we need beamtime to collected MAD or SAD datasets to determine the first usher structure.