CONSTRUCTION OF A FLEXIBLE HYPERPOLARIZATION SYSTEM

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 在RR正在建造一台灵活的动态核极化仪器。它计划成为一种比商业上可用的牛津超级感测DNP系统更灵活的仪器。此外，购买HyperSense的条款和条件禁止在体内进行实验。着眼于大型哺乳动物在体内的潜在应用，该系统正在围绕一个更大的样本容器进行设计，该容器将容纳大约两倍于HyperSense样本杯的样本量。DNP系统将基于与华盛顿大学马克·康拉迪合作设计的低温恒温器，放置在布鲁克15厘米口径的磁铁中，磁场为~4.6特斯拉。最近的结果表明，场强的递增可以产生显著更高的核极化。(1，2)考虑到这一点，我们设计了新的系统，工作在~129 GHz的ESR频率。此外，大口径磁铁中可用的额外空间应允许1H和13C或89Y的双共振探头。交叉极化固态核磁共振脉冲序列可以用来将极化从高伽马质子转移到探测核，高伽马质子在DNP下的极化速度比低伽马粒子快得多。(3)构建偏振器的参考文献：1.Jannin S，评论A，Kurdzesau F，Konter JA，Hautle P，van den Brandt B，van der Klink JJ。用于溶解动态核极化的140 GHz预偏振器。《化学物理杂志》2008年；128(24)：241102-241104。2.J？hannesson H，Macholl S，Ardenkjaer-Larsen JH.4.6特斯拉下[1-13C]丙酮酸的动态核极化《磁共振杂志》；197(2)：167-175。3.Maly T，DebelouChina GT，Bajaj vs，Kan-Nian H，Chan-Gyu J，Mak-Jurkoskas ML，Sirigiri Jr，van der Wel PCA，Herzfeld J，Temkin RJ，Griffin RG。强磁场下的动态核极化。化学物理杂志2008；128(5)：N.PAG。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. A flexible dynamic nuclear polarization instrument is under construction at the RR. It is planned to be a more flexible instrument than the commercially-available Oxford HyperSense DNP system. Further, the terms and conditions of purchase of the HyperSense prohibit in vivo experiments. With an eye towards potential large mammal in vivo applications, the system is being designed around a larger sample container that will hold approximately twice the amount of sample as the HyperSense sample cup. The DNP system will be based on a cryostat designed in collaboration with Mark Conradi of Washington University placed in a Bruker 15cm bore magnet energized to a field of ~4.6 Tesla. Recent results have demonstrated that incremental increases in field strength can yield dramatically higher nuclear polarizations.(1,2) With this in mind, we have designed the new system to operate at an ESR frequency of ~129 GHz. Furthermore, the extra space available in the large bore magnet should allow the incorporation of a double resonance probe for 1H and either 13C or 89Y. Cross-polarization solid state NMR pulse sequences can then be used to transfer polarization from high gamma protons, which polarize much more quickly under DNP than lower gamma species, to the detection nucleus.(3) References for construction of a polarizer: 1. Jannin S, Comment A, Kurdzesau F, Konter JA, Hautle P, van den Brandt B, van der Klink JJ. A 140 GHz prepolarizer for dissolution dynamic nuclear polarization. The Journal of Chemical Physics 2008;128(24):241102-241104. 2. J¿hannesson H, Macholl S, Ardenkjaer-Larsen JH. Dynamic Nuclear Polarization of [1-13C]pyruvic acid at 4.6 tesla. Journal of Magnetic Resonance 2009;197(2):167-175. 3. Maly T, Debelouchina GT, Bajaj VS, Kan-Nian H, Chan-Gyu J, Mak-Jurkauskas ML, Sirigiri JR, van der Wel PCA, Herzfeld J, Temkin RJ, Griffin RG. Dynamic nuclear polarization at high magnetic fields. Journal of Chemical Physics 2008;128(5):N.PAG.