SUSCEPTIBILITY AND DIFFUSION CONTRAST AT HIGH MAGNETIC FIELD

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者的研究机构。 我们感兴趣的是在高磁场下小鼠大脑中的可感知性变化所产生的磁刺激的成像和定量。这项工作是基于Duyn等人的观察，即大脑的细胞结构细节在相位对比图像中比传统的对比度指标（例如T1、T2、扩散）更加明显。我们相信这是一个从根本上使项目，将允许扩展到许多不同的领域。该项目有几个不同的阶段： 第一阶段：我们将建立基于卢卡斯中心（斯坦福大学）先前所做工作的相位对比成像方法。这将涉及测量和校正7 T MR系统中的Bo不均匀性，以及测量和校正M2M 35 mm正交线圈的B1不均匀性。 第2阶段：我们将使用相衬方法的3D实现以< 100微米的空间分辨率对活的C57 BL/6 J小鼠进行成像。我们将比较大脑特定区域的对比度与更传统的成像协议（SPGR，FSE和FIESTA） 第三阶段：我们将该方法导出到9.4T系统，以允许在21微米处对灌注固定的大脑进行成像，其中活性染色剂（ProHance）的分布可以提供更显著的相位对比。 第4阶段：我们将研究检测小浓度SPIO造影剂的可能性 最初在体模中进行，以确定分子成像的潜在灵敏度增强。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. We are interested in imaging and quantifying the constrast generated by suceptibility variations in the mouse brain at high magnetic field. The work is based on the observations by Duyn et al that cytoarchitectural details of the brain can become much more apparent in phase contrast images than with more traditional contrast metrics (e.g. T1,T2, diffusion) . We believe this is a fundamentally enabling project that will allow extension into many different areas. The project has several different phases: Phase 1: We will establish the method for phase contrast imaging based upon work done previously at the Lucas Center (Stanford). This will involve measuring and correcting for Bo inhomogenities in the 7T MR system and measuring and correcting for B1 inhomogenoty of the M2M 35 mm quadrature coil. Phase 2: We will image live C57BL/6J mice using a 3D implementation of the phase contrast method at spatial resolution of < 100 microns. We will compare the contrast seen in specific regions of the brain to more traditional imaging protocols (SPGR, FSE, and FIESTA) Phase 3: We will export the method to the 9.4T system to allow imaging of perfusion fixed brains at 21 microns where distribution of the active stain (ProHance) may provide more dramatic phase contrast. Phase 4: We will look toward to possibility of detecting small concentrations of SPIO contrast agent initally in phantoms to determine the potential sensitivity enhancement for molecular imaging.