MEASURING CELL REFRACTIVE INDEX BY CONFOCAL REFLECTANCE AND HP MICROSCOPY

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 定量位相测量实现了独特的稳定和高分辨率的位相测量，它可以与现有的光学显微镜对接用于生物医学研究，这是一个吸引人的特点。这种无标记的定量时相测量使我们能够在没有扰动的情况下研究活细胞。然而，这项技术只提供了总的相位，即折射率和光束通过单元的路径的线积分。这项工作表明，结合共焦反射和定量位相显微镜可以更好地估计活细胞的平均折射率。利用共焦反射率得到细胞的轮廓，用希尔伯特位相显微镜获得细胞引起的相移，不需要假设或约束细胞的几何形状，就可以确定呈现蛋白质含量(干重)的细胞的平均折射率。这种方法可以为原位无标记活细胞提供新的生物检测方法。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Quantitative phase measurements have achieved uniquely stable and high resolution measurements of phase and it becomes the appealing feature that they can be interfaced with an existing optical microscope for biomedical research. This label-free quantitative phase measurement allows us to study live cell without perturbation. However the technique offers only a total phase that is the line integral of the refractive index and the light beam's path through a cell. This work demonstrates a combining confocal reflectance and quantitative phase microscopy can be used to better estimate an average refractive index of living cell. Using the confocal reflectance to obtain the cell contour and the Hilbert phase microscopy to obtain the phase shift induced by the cell, the average refractive index of cell which presents protein contents (dry-mass) can be determined without postulating or constraining the cell to a known geometry. This approach can provide novel biological assays of label-free living cells in situ.