STRUCTURE OF A POTASSIUM ION TRANSPORTER

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 离子转运体是一种完整的膜蛋白，它共同运输两种不同的离子物种穿过膜。它们在人体生理中扮演着重要的角色：例如，钠/碘同向转运体负责在甲状腺中积累碘。然而，缺乏对符号如何工作的原子层面的机械理解。这项研究的长期目标是获得大肠杆菌钠/钾同向转运体TrkH的高分辨结构，该结构与调节细胞内蛋白TrkA形成复合体。我们将解决如何实现离子选择性，以及细胞内ATP水平如何调节离子转运功能的问题。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Ion symporters are integral membrane proteins that co-transport two different ion species across the membrane. They play important roles in human physiology: the Sodium/Iodide symporter, for example, is responsible for accumulation of iodide in the thyroid gland. However, an atomic level mechanistic understanding of how symporters work is lacking. The long term goal of this research is to obtain high resolution structures of an E. coli Sodium/Potassium symporter, TrkH, in complex with a regulatory intracellular protein, TrkA. We will address the questions of how the ion selectivity is achieved, and how the function of ion transport is regulated by intracellular ATP level.