USER ORIENTATION, TRAINING AND SUPPORT FOR BIO-SAXS/D DATA ACQUISITION AND DATA

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 SSRL BTP工作人员为用户提供有效使用SMB SAXS/D仪器的实际操作培训。 用户首先会收到针对BL 4 -2的简短安全指导和最近设备变更的更新。用户将收到一个关于Blu-ICE的简短教程，用于大多数非晶体散射和衍射实验。他们还接受了使用MarParse的培训，MarParse是BTP员工最近开发的解决方案分散的数据处理程序，以及其GUI版本MarParseDlg。用户被指示在解决方案的X射线散射数据分析的基础知识，如检查数据可能的辐射诱导的聚集，减去背景，并计算回转半径。数据处理程序Fit 2D，在欧洲同步辐射设施，以及从EMBL-Hamburg的Primus开发的也包括在要求。介绍教程复杂的解决方案散射数据解释软件包，由博士Svergun（EMBL汉堡）开发，涵盖。用户建议在实验几何形状，样品处理设备和数据收集策略，特别是最大限度地减少辐射损伤，以帮助提高数据质量的实际实验方面。 这些是给新用户组，并已发现有用的经验丰富的用户也。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The SSRL BTP staff provides user hands-on training for the effective use of the SMB SAXS/D instrument. Users first receive a brief safety orientation specific to BL4-2 and updates on recent equipment changes. Users receive a short tutorial on Blu-ICE used for the majority of non-crystalline scattering and diffraction experiments. They also receive training on the use of MarParse, the data processing program for solution scattering developed recently by the BTP staff, as well as its GUI version MarParseDlg. Users are instructed on the basics in solution x-ray scattering data analysis, such as inspecting data for possible radiation-induced aggregation, subtracting the background, and calculating radii of gyration. The data processing program Fit2D, developed at the European Synchrotron Radiation Facility, as well as Primus from EMBL-Hamburg are also covered upon request. Introductory tutorials to the sophisticated solution scattering data interpretation software packages, developed by Dr. Svergun (EMBL-Hamburg), are covered. Users are advised on practical experimental aspects on experiment geometries, sample-handling equipment, and data collection strategies especially for minimizing radiation damage to help improve data quality. These are given to new user groups and have been found useful also for experienced users.