CRYSTALLOGRAPHIC STUDIES OF RECEPTORS OF NEUROLOGICAL AND IMMUNOLOGICAL RELEVANC

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 我的实验室对与人类健康和疾病相关的细胞-表面受体/配体相互作用的结构方面感兴趣。通过结构分析，我们希望回答有关跨膜信号转导的基本机制问题，并产生可作为药物设计模板的结构数据。我们致力于大量糖蛋白受体的表达和结晶研究，既有单独的，也有与其配体形成的复合体。我们既表达了可溶性受体结构域，也表达了全长完整的膜蛋白。我们研究的受体系统主要是在免疫和神经系统中，特别强调了解分子识别中的交叉反应。我们的大多数项目严重依赖于在同步加速器设备上收集X射线数据的能力，因为我们可以生长出大型和异质蛋白质复合体的晶体，通常质量较小和中等。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. My laboratory is interested in structural aspects of cell-surface receptor/ligand interactions with relevance to human health and disease. Through structural analysis we hope to answer basic mechanistic questions about transmembrane signaling, as well as produce structural data that can be used as a template for drug design. We are engaged in expression and crystallization studies of numerous glycoprotein receptors both alone and in complex with their ligands. We are expressing both soluble receptor domains as well as full-length integral membrane proteins. The receptor systems we study are primarily in the immune and nervous systems, with a special emphasis on understanding cross-reactivity in molecular recognition. Most of our projects we are critically dependent on the ability to collect x-ray data at a synchrotron facility due to the generally small and moderate quality of the crystals which we can grow of large and hetero-geneous protein complexes.