LOUISIANA COBRE: OBESITY & DIABETES RES: CELL BIOLOGY & BIOIMAGING CORE

这个子项目是许多利用 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 细胞生物学和生物成像（CBB）核心（http：//labs.pbrc.edu/cellbiology/index.htm）是根据COBRE项目PI和彭宁顿生物医学研究中心研究人员的具体需求组织的。 其目的是开发和提供最先进的生物成像方法，以研究活细胞和固定细胞和组织中生物过程的细胞和分子生物学，以及使用微型CT的体内生理过程。 CBB核心提供最先进的生物成像和分析仪器和支持，以促进COBRE项目PI和PBRC教师的数据收集和分析。 CBB核心由大卫H伯克博士领导，他担任主任和两名研究助理  劳拉·罗恩女士和考特尼·凯恩女士。 2006年9月，对彭宁顿COBRE的资助使细胞生物学和生物成像核心得以存在，它围绕着五个主要的分析平台组织起来：蔡司LSM-510 Meta光子共聚焦，直立式荧光显微镜和倒置式荧光显微镜，用于活细胞成像的扫描荧光计，以及FACS Caliber分析细胞分选仪。 最初两年的主要重点是加强我们现有的显微镜专业知识，并在流式细胞术，免疫组织化学，成像和图像分析领域开发新的专业知识。我们的总体目标是通过提供无缝访问这些技术来提高我们教师的竞争力。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The Cell Biology and Bioimaging (CBB) Core (http://labs.pbrc.edu/cellbiology/index.htm) was organized in response to the specific needs of COBRE Project PIs and researchers at the Pennington Biomedical Research Center. Its purpose is to develop and provide state of the art bioimaging approaches to study the cell and molecular biology of biological processes in live and fixed cells and tissues, as well as physiological processes in vivo using micro CT. The CBB Core provides state of the art bioimaging and analytical instrumentation and support to facilitate data collection and analysis by COBRE Project PIs and PBRC faculty. The CBB Core is led by Dr David H Burk who serves as director and two research associates  Ms Laura Roan and Ms Courtney Cain. Funding of the Pennington COBRE in September 2006 brought the Cell Biology and Bioimaging Core into existence and it was organized around five major analytical platforms: a Zeiss LSM-510 meta multiphoton confocal, an upright epifluorescence microscope and an inverted epifluorescence microscope, a scanning fluorometer for live cell imaging, and a FACS Caliber analytical cell sorter. A primary focus in the first two years was to enhance our existing microscopy expertise and development new expertise in the areas of flow cytometry, immunohistochemistry, imaging and image analysis. Our overall goal is to enhance the competitiveness of our faculty by providing seamless access to these technologies.