MICROBIAL ANALYSIS OF DENTAL PLAQUE SPECIES IN THE SEA ISLAND GULLAH POPULATION

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 在最近一项对Gullah 2型糖尿病患者龋齿的研究中，没有发现糖尿病的血糖控制与龋齿之间的联系，无论是否经过吸烟、BMI、年龄和性别的调整。正如预期的那样，古拉族人口的平均龋齿缺失充填分数(DMFT)高于NHANES白人人口。然而，与1989年国民健康保险黑人(12.43±0.14)分和1999年国民健康保险黑人(11.07±0.018)分相比，古拉族人口的DMFT得分也更高(17.66.9分)。4.与国民健康保险黑人人口相比，古拉族人口的DMFT得分更高，这表明除了收入和获得护理的机会外，其他因素也参与了这一人群的牙病发病。需要考虑的一个因素是古拉族人口口腔微生物区系的差异。已知的口腔病原体的类似研究也在9-14岁的一般人群中进行，但到目前为止，文献中还没有关于Gullah人群的这种研究。这项初步研究的目的是用棋盘DNA-DNA杂交法(Forsythe Institute)鉴定Gullah 2型糖尿病患者牙菌斑中的口腔微生物种类。为研究更多革兰氏阳性致龋菌而开发的改良棋盘格板将被使用9。选择细菌种的个体的百分比将与非Gullah 2型糖尿病的类似研究进行比较14。所获得的信息将阐明Gullah人群特有的潜在口腔病原体，并为该人群的牙病管理制定方案。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. In a recent study of dental caries in Gullah type 2 diabetics, no association was found between glycemic control of diabetes and dental caries both with and without adjustment for smoking, BMI, age and sex8. As expected the mean Decayed-Missing-Filled Tooth (DMFT) scores for the Gullah population was higher than that of the NHANES white population. However, the DMFT scores for the Gullah population was also higher (17.6¿6.9) as compared to both the 1989 NHANES Blacks (12.43¿0.14) and 1999 NHANES Blacks (11.07¿0.018) 4. Higher DMFT scores in the Gullah population as compared to the NHANES Black population suggests other factors in addition to income and access to care are involved in the pathogenesis of dental disease in the this population. One factor to consider is the difference in the oral microbial flora of the Gullah population. Similar studies of known oral pathogens have been conducted on the general population 9-14, but to date no such study on the Gullah population exists in the literature. The purpose of this pilot study is to characterize the oral microbial species in the supra-gingival dental plaque of Gullah type 2 diabetics using checkerboard DNA-DNA hybridization (Forsythe Institute). A modified checkerboard panel developed to study the more gram positive cariogenic species will be used 9. The percent of individuals that have selected bacterial species present will be compared to similar studies of non-Gullah type 2 diabetics14. Information gained will illuminate potential oral pathogens unique to the Gullah population as well as generate protocols for the management of dental disease in this population.