Stability of cross-linked dentin and its clinical application

描述(由申请者提供)：这项建议的长期目标是为Ana Karina Bedran-Russo博士提供机会，使其发展成为一名准备充分、有竞争力和独立的年轻科学家，将积极的研究生涯与教学和实践相结合。申请者的最终目标是通过为翻译研究做出贡献来改善患者的护理。在K08指导临床医生科学家奖期间获得的组织工程、口腔生物学和生物材料方面的额外培训将在支持性的多学科环境中为牙科学术生涯提供必要的技能，使Bedran-Russo博士迅速转变为一名独立的临床科学家。她的研究和培训将在詹姆斯·德拉蒙德博士和安妮·乔治博士的指导下进行。申请者将在这项研究的基础上寻求额外的资金。这项拨款的研究部分旨在调查胶原蛋白的生物化学修饰对牙本质特性的贡献，从而对粘附性修复牙科治疗的耐久性做出贡献。复合树脂修复的后牙平均寿命为7年，此时界面失效是更换的主要原因，占所有替换修复的30-60%。I型胶原纤维与牙本质粘结时的完整性是一个重要的问题，因为它是修复过程中涉及的主要牙齿成分。需要检验的假设是，牙本质中人工形成的胶原交联物将导致修复界面具有更好的机械性能，以抵御随着时间的推移而降解。提出的具体目标是：目的1.测定不同交联剂处理后牙本质的生化性质。在短期和长期评估中，将对健全的和受龋齿影响的人牙本质进行生化特性(胶原蛋白交联分析-高效液相和免疫组织化学分析)的评估。目的2：在短期和长期评估中评估处理后牙本质(声音和龋齿影响)的机械性能。硬度和硬度将在特定部位使用原子力显微镜进行评估。牙本质的极限强度和牙本质/修复体界面的粘结强度将使用通用试验机进行评估。这些生物化学和机械相结合的方法将为牙本质胶原蛋白的质量和修复体的长期耐久性提供深入的见解。

DESCRIPTION (provided by applicant): The long term goal of this proposal is to provide Dr. Ana Karina Bedran-Russo, DOS, MS, PhD with the opportunity to develop into a well prepared, competitive and independent young scientist that will combine an active research career with teaching and practice. The applicant's ultimate goal is to improve patient care by contributing to translational research. Additional training in tissue engineering, oral biology and biomaterials obtained during this K08 Mentored Clinician Scientist Award will provide the necessary skills for a dental academic career track within a supportive multidisciplinary environment for the rapid transition of Dr. Bedran- Russo to an independent clinician scientist. Her research and training will be conducted under the guidance of Dr. James Drummond and Dr. Anne George. The applicant will build upon this research to seek additional funding. The research component of this grant aims to investigate the contribution of biochemical modifications to collagen on the properties of dentin and consequently on the durability of adhesive restorative dental treatments. A composite resin restoration last an average of 7 years in posterior teeth at which time failure at the interface is the primary reason given for replacement and accounts for 30-60% of all replaced restorations. The integrity of fibrillar type I collagen is an important issue when bonding to dentin since it is the main tooth component involved during restorative procedures. The hypothesis to be tested is that the artificially formed collagen cross-links in dentin will result in restoration interfaces with improved mechanical properties to withstand degradation over time. The specific aims proposed are: Aim 1. Determine the biochemical properties of dentin following treatment with different crosslinking agents. Biochemical properties (collagen cross-linking analysis - HPLC and immunohistochemical analysis) will be assessed in sound and caries-affected human dentin in both short and long term evaluations. Aim 2: Assess the mechanical properties of the treated dentin (sound and caries-affected) at short-term and long-term evaluations. Stiffness and hardness will be evaluated in specific sites using an atomic force microscope. Ultimate strength of dentin and bond strength of the dentin/restoration interface will be assessed using a universal testing machine. These combined biochemical and mechanical approaches will provide insights to correlate quality of dentin collagen and the long-term durability of restorations.