DNA Sequencing

DNA测序和分析核心在过去的21年里一直在运行，并 为CFAR成员提供最先进的自动测序(DNA测序设施)和 计算机分析能力(分子和遗传生物信息学设施)。核心功能有 包括为自动测序过程开发新的方法，培训 研究人员使用序列分析工具，开发新的分子生物学方法来 确定艾滋病毒/艾滋病毒遗传多样性的特征，并支持多学科和多机构项目 和合作。在上一个预算期，DNA测序设施支持了62个CFAR 调查人员，并为100多项与艾滋病有关的赠款和合同提供基本服务。1.94亿 确定了初级序列的碱基对，产生了超过210万美元的用户退款。 最近实施的自动化提高了核心效率，并允许减少用户的按存储容量使用计费 每个测序反应从8美元到6美元不等。在同一时间段，分子和遗传学 生物信息学基金积极支持了30多名CFAR调查人员，并提供了基本服务 用于62项与艾滋病有关的赠款和合同。核心的具体目标是： 1.继续为CFAR成员提供自动DNA测序能力 专用毛细管生物系统DNA测序仪的可用性和维护 电泳仪。 2.建立新的方法、技术和试剂，以协助调查人员进行表征 艾滋病毒/SIV基因多样性。 3.提供和维护一套全面的现代生物信息数据库和分析 用于分析遗传信息的工具，包括基因和基因组序列。 4.在使用这些生物信息资源方面提供技术支持和培训。

The DNA Sequencing and Analysis Core has been in operation for the past twenty-one years and has provided CFAR members with state-of-the-art automated sequencing (DNA Sequencing Facility) and computer analysis capabilities (Molecular and Genetic Bioinformatics Facility). Core functions have included development of new methodologies for the automated sequencing process, training of investigators in the use of sequence analysis tools, development of new molecular biology approaches to characterize HIV/SIV genetic diversity, as well as supporting multidisciplinary and multi-institutional projects and collaborations. In the last budget period, the DNA Sequencing Facility has supported 62 CFAR investigators and provided essential services for over 100 AIDS related grants and contracts. 194 million base pairs of primary sequence were determined, generating over $2,100,000 in user charge-backs. Recently implemented automation has increased core efficiency and allowed to reduce user chargebacks from $8 to $6 per sequencing reaction. During the same time period, the Molecular and Genetic Bioinformatics Facility has actively supported over 30 CFAR investigators, and provided essential services for 62 AIDS related grants and contracts. Specific Aims of the Core are: 1. To continue to provide automated DNA sequencing capabilities to CFAR members through the availability and maintenance of dedicated Applied Biosystems DNA Sequencers with capillary electrophoresis systems. 2. To establish new methods, technologies and reagents to assist investigators in the characterization of HIV/SIV genetic diversity. 3. To provide and maintain a comprehensive set of modem bioinformatic databases and analytical tools for the analysis of genetic information, including gene and genomic sequences. 4. To provide technical support and training in the use of these bioinformatic resources.