NMSU CELL AND ORGANISM CORE FACILITY

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 新墨西哥州立大学的细胞和有机体核心设施(COCF)提供了一个设施，可以利用NM Brin/INBRE赠款的资金进行组织培养实验。它是INBRE和非INBRE资助的初级教员研究计划的关键资产，它为研究人员和学生创建了一个研究基础设施，用于使用原代细胞培养、来自各种生物的活组织外植体准备和建立的细胞系进行研究。COCF还促进社区中非大学科学家对其的使用。COCF目前支持4个积极的研究项目，涉及学生、教职员工和教职员工。这些研究包括：1)细胞质分裂和细胞周期调控(Shuster)的分子机制；2)脊椎动物(Unguez)再生过程中细胞去分化和转分化的分子机制；3)原代培养的硬骨脊椎动物S.macrurus(Unguez)卫星细胞的建立；4)细胞内转导新机制(Smirnov)的发展。COCF正在推动日常研究活动，不仅涉及教职员工，还涉及他们的研究生和本科生。在过去的12个月里，共有12名学生(6名研究生，6名本科生)接受了细胞培养技术的培训，这些学生制定了优化的组织特异性体外研究方案。该设施的进一步成熟将纳入更广泛的项目，并支持更多NMSU研究人员和来访科学家的需求。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The Cell and Organism Core Facility (COCF) at New Mexico State University provides a facility equipped to carry out tissue culture experiments using funds from the NM BRIN/INBRE grant. It is a critical asset to the research programs of INBRE and non-INBRE funded junior faculty by creating a research infrastructure for investigators and students to perform studies using primary cell cultures, live tissue explant preparations from a variety of organisms, and established cell lines. The COCF also promotes its use by non-university scientists in the community. The COCF currently supports 4 active research projects that involve students, staff and faculty. These include: 1) molecular mechanisms involved in the timing of cytokinesis and cell cycle regulation (Shuster) 2) cell dedifferentiation and transdifferentiation during regeneration in vertebrates (Unguez), 3) establishment of primary satellite cell culture from a teleost vertebrate S. macrurus (Unguez), and 4) development of new mechanisms of intracellular transfection (Smirnov). The COCF is enabling daily research activities involving not only faculty, but their graduate and undergraduate students as well. In the past 12 months, a total of twelve students (6 graduate, 6 undergraduate) were trained in cell culture techniques and these students developed protocols optimized for tissue-specific in vitro studies. Further maturation of the facility will incorporate a broader range of projects and support the needs of additional NMSU researchers and visiting scientists.