NOVEL SIDEROPHORES PRODUCED BY MARINE BACTERIA AND FUNGI

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 背景：许多微生物产生被称为铁载体的低分子铁(III)特异性结合化合物来竞争铁。微生物被认为能产生独特的铁载体结构，从而成功地与环境中的其他生物竞争铁。从陆地细菌和真菌以及从海洋细菌中分离出来的铁载体在生物医药和控制微生物生长方面显示出很好的前景。海洋真菌产生的铁载体还没有被表征，因此提供了一种新的生物活性化合物的未知来源。 假设：海洋来源的真菌产生具有新结构的铁载体。 方法：在铁限制的条件下，在纯培养条件下筛选海洋来源的真菌菌株以产生铁载体。铁载体的分离和结构表征使用氨基酸分析、脂肪酸分析、核磁共振、质谱分析和其他技术，视情况而定。 结果和未来方向：目前，我们已经鉴定出秀丽坎宁汉姆杆菌ATCC 36112是一种产生海洋来源真菌的铁载体。从该真菌中分离到一个新的铁载体，并用紫外-可见光谱、电喷雾电离质谱仪和串联质谱仪对其进行了表征。我们目前正在努力提纯足够的材料进行核磁共振分析，这将使我们能够完成这个新的铁载体的结构确定。我们还开始筛选几种来自海洋的酵母菌株。这些菌株似乎产生铁载体(基于Fe(III)-CAS分析)，我们将立即开始提纯化合物。计划在不久的将来进行的实验将分离这些新菌株产生的铁载体并对其结构特征进行表征。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Background: Many microorganisms produce low-molecular-weight, iron(III)-specific binding compounds called siderophores to compete for iron. Microbes are thought to produce unique siderophore structures to successfully compete for iron against other organisms in their environment. The siderophores isolated from terrestrial bacteria and fungi and from marine bacteria show promise as biomedical agents and for control of microbial growth. The siderophores produced by marine fungi have not been characterized and thus provide an unexplored source of novel bioactive compounds. Hypothesis: Marine-derived fungi produce siderophores with novel structures. Methods: Marine-derived fungal strains are screened for production of siderophores in pure cultures under conditions of iron limitation. Siderophores are isolated and structurally characterized using amino acid analysis, fatty acid analysis, NMR, mass spectrometry, and other techniques, as appropriate. Results and future directions: At this time, we have identified Cunninghamella elegans ATCC 36112 as a siderophore producing marine-derived fungus. A novel siderophore has been isolated from the fungus and characterized using UV-Visible spectrophotometry, electrospray ionization mass spectrometry, and tandem mass spectrometry. We are currently working to purify sufficient material for analysis by NMR, which will allow us to complete the structural determination for this novel siderophore. We have also begun screening several strains of marine-derived yeasts. These strains appear to produce siderophores (based on Fe(III)-CAS assay) and we will begin purification of the compounds immediately. Experiments planned in the immediate future will isolate and structurally characterize siderophores produced by these new strains.