Flow Cytometry Core

流式细胞术核心的任务是协助研究人员执行多参数分析和基于荧光偶联抗体或其他荧光染料试剂标记的细胞群的高速分选。除了为调查人员提供先进的流式细胞仪设备和技术专长外，该中心还提供培训，指导DRTC调查人员及其工作人员应用基本和先进的流式细胞仪技术。该中心提供的分析性和预备性细胞分析使DRTC研究人员能够确定与人类和啮齿动物模型的代谢功能障碍相关的细胞群体的具体变化。Flow细胞仪核心的专业技术和知识渊博的员工为研究人员提供了广泛的专业、高质量的方法和工具，这些方法和工具与了解与代谢调节相关的适应性和先天免疫系统以及发育和再生研究相关，这些研究现在被认为是糖尿病和肥胖症的重要组成部分。为了实现这些目标，流式细胞术核心将：1)向研究人员、受训人员和工作人员提供关于针对其特定研究问题使用FACS的建议；2)为研究人员提供专门的流式细胞术分析，以量化表达特定表面标记或经历特定细胞信号事件的细胞；3)提供高速基于流动的荧光激活细胞分选，用于无菌分离显示特定细胞表面标记或生物学特性的细胞；4)为学生、博士后研究员、研究人员和技术人员提供实验室培训，以执行基于荧光的细胞分析和细胞分类所需的技术；以及5)协助进行适当的数据分析，以评估分子靶标和生物过程。所有这些服务都提供给糖尿病研究的新手，以及从事糖尿病相关项目的研究人员，这些项目可以通过使用这一核心的专门知识和设施得到丰富和扩展。

The mission of the Flow Cytometry Core is to assist investigators in performing multi-parameter analysis and high speed sorting of cell populations based on labeling with fluorochrome conjugated antibodies or other fluorescent dye reagents. In addition to providing advanced flow cytometry equipment and technical expertise for investigators, the Core also provides training to guide DRTC investigators and their staff in the application of basic and advanced flow cytometry techniques. The analytical and preparative cell analyses provided by this Core allows DRTC investigators to identify specific changes in cell population that are associated with metabolic dysfunction in human and rodent models. The expertise and knowledgeable staff of the Flow Cytometry Core provides investigators a wide range of specialized, high quality methodologies and tools relevant to understanding the adaptive and innate immune systems related to metabolic regulation as well as developmental and regenerative studies that are now recognized as important components of diabetes and obesity. To accomplish these goals, the Flow Cytometry Core will: 1) advise investigators, trainees and staff on the utility of FACS for their specific research problem; 2) make available to investigators specialized flow cytometry analyses for the quantification of cells expressing specific surface markers or undergoing specific cellular signaling events; 3) make available high-speed flow-based flourescence activated cell sorting for the sterile isolation of cells displaying specific cell surface markers or biological properties; 4) provide laboratory training of students, postdoctoral fellows, investigators and technical staff in performing techniques necessary to perfonn fluorescent-based cell analyses and cell sorting; and 5) assist in the appropriate data analysis for the evaluation of molecular targets and biological processes. All these services are available to investigators new to diabetes research, as well as to investigators working on diabetes-related projects that can be enriched and extended by the use of the expertise and facilities of this core.