Early Detection Of Renal Injury

1)继续我们将外切体作为生物标志物来源的工作，我们在患者的唾液外切体中发现了microRNA。MicroRNAs可以在转录后调节大量基因，这补充了我们在外体中转录因子的工作。我们继续在更大的患者组和动物模型中精炼这两种有前途的生物标志物候选对象。 2)临床上将血清肌酐作为急性肾损伤的生物标志物，并对小鼠脓毒症后摘除肾脏的效果进行了测试，结果发现血清肌酐水平下降。这与预期的效果相矛盾，我们探索了可能的原因。我们发现脓毒症时循环肌酐的净产量减少。这一观察结果现在可以解释为什么血清肌酐的如此小的增加仍然低估了脓毒症患者的肾脏损伤。我们继续深入研究这一现象的机制(S)。

1) Continuing our work on exosomes as a source of biomarkers, we found microRNA in human salivary exosomes from patients. MicroRNAs can regulate large numbers of genes post-transcriptionally, which complements our work with transcription factors in exosomes. We continue refinement of both types of promising biomarker candidates in larger sets of patients, as well as in animal models. 2) Serum creatinine is used clinically as a biomarker for acute kidney injury, and we tested the effect of removing the kidneys after sepsis in mice, and we were surprised to find that serum creatinine decreased. This contradicts the expected effect, and we explored possible causes. We found that net production of circulating creatinine is decreased during sepsis. This observation can now explain why such small increases in serum creatinine still underestimate kidney injury in sepsis patients. We continue to delve into the mechanism(s) for this phenomenon.