Early Detection Of Renal Injury

肾脏疾病很难检测，特别是急性肾损伤（也称为急性肾衰竭）。我们正在开发新的方法来检测肾脏疾病，包括MRI，尿液或血液检查。 1)使用树枝状大分子钆螯合物纳米颗粒在小鼠MRI中检测近端小管损伤。我们发现钆纳米颗粒在近端小管中积累，并且可以用于检测肾脏结构、功能和损伤。我们将败血症急性肾损伤的早期检测和结果预测方法扩展到其他形式的肾损伤，包括慢性肾脏疾病。 2)早期诊断的标志物。我们是新的使用微阵列和蛋白质组学技术，以寻找缺血/再灌注，肾毒性和脓毒症引起的急性肾损伤的早期生物标志物。我们有一些优秀的候选者，包括胎球蛋白A，正在使用我们的小鼠和大鼠急性肾损伤模型进行验证。 3)我们已经制定了收集，储存和处理条件，使用尿外泌体蛋白作为肾脏疾病的生物标志物。我们开始在我们的动物模型中寻找结构性肾损伤的外泌体标志物。

Renal disease is difficult to detect, particularly in a form called acute kidney injury (also known as acute renal failure). We are developing new methods to detect renal disease involving either MRI, or urine or blood tests. 1) Detection of proximal tubule damage in mice MRI using dendrimer gadolinium chelate nanoparticles. We found that Gadolinium nanoparticles accumulate in the proximal tubule, and can be used to detect renal structure, function, and injury. We extending our methods for the early detection and outcome prediction of sepsis-acute kidney injury to other forms of kidney damage, including chronic kidney disease. 2) Markers for early diagnosis. We are new using microarray and proteomic techniques to search for early biomarkers of ischemia/reperfusion-, nephrotoxic-, and sepsis-induced acute kidney injury. We have a few excellent candidates, including Fetuin A, that are being validated using our mouse and rat acute kidney injury models. 3) We have worked out the collection, storage, and processing conditions to use urinary exosomal proteins as biomarkers of renal disease. We are beginning to search for exosomal markers of structural renal injury in our animal models.