Transmission Electron Microscope for a Shared Core Facility

描述（由申请人提供）：此资助申请要求购买FEI Technai G2精神生物双透射电子显微镜的资金。需要这种基本仪器来替换维克森林大学医学院唯一共用显微镜核心设施中老化且不可靠的>30年的Philips 400 TEM。这种新仪器具有计算机控制的操作系统，数字成像相机，以及高达120 kV的加速电压，可以对生物标本进行高分辨率TEM成像。空间，管理，经验丰富的技术人员和机构对这个共享的显微镜核心的支持已经存在了20多年，并支持许多不同部门的几个NIH资助的研究人员的研究，包括本申请中列出的10名研究人员作为当前的主要用户。除了TEM仪器外，核心设施还包括现有的SEM仪器和基于光的显微镜仪器，包括数字荧光，共聚焦，延时和激光捕获显微切割显微镜，所有这些都是TEM样品分析的强相关工具。该核心提供TEM支持准备服务，包括包埋和薄切片，冷冻切片，固定角度和旋转阴影和负染色，以及免疫细胞化学技术。这种新型显微镜的收购对于提供最先进的仪器以继续支持NIH资助的研究人员的显微镜需求至关重要，并提高了这些和新研究项目的灵活性和可靠性。

DESCRIPTION (provided by applicant): This grant application requests funds for the purchase of an FEI Technai G2 Spirit Bio- Twin transmission electron microscope. This essential instrument is needed to replace an aged and undependable >30 year old Philips 400 TEM in the sole shared-use microscopy core facility in the Wake Forest University School of Medicine. This new instrument has computer-controlled operating systems, a digital imaging camera, and up to 120kV accelerating voltage to allow high resolution TEM imaging of biological specimens. Space, administration, highly-experienced technicians and institutional support for this shared microscopy core have been in place for over 20 years, and support the research of several NIH grant-funded investigators in many different departments, including a group of 10 investigators listed in this application as current major users. The core facility includes, in addition to the TEM instrument, an existing SEM instrument and light-based microscopy instruments, including digital fluorescence, confocal, time-lapse and laser-capture microdissection microscopes, all of which serve as strong correlative tools for the analysis of TEM samples. The core provides TEM support preparative services, including embedding and thin sectioning, cryoultramicrotomy, fixed angle and rotary shadowing and negative staining, as well as immunocytochemical techniques. The acquisition of this new microscope is critical to provide a state-of-the art instrument to continue the support of microscopy needs for NIH-funded investigators, with improved flexibility and dependability for these and for new research projects.