ABI Elite ESI-QqTOF Mass Spectrometry System

描述（由申请人提供）：我们申请资金购买液相色谱-电喷雾电离质谱（LC-ESI-MS）集成系统。该系统包括能够快速扫描和高精度质量测量的Applied Biosystems（ABI）QStar Elite四极杆飞行时间（QqTOF）质谱仪，以及用于高分辨率分析物分离的沃茨超高压液相色谱系统。拟议的仪器将取代目前的ABI QStar Pulsar QqTOF质谱仪，该质谱仪必须在2010年7月1日退役，届时ABI将终止当前仪器的所有维修和服务。该系统代表了中央分析设施的关键仪器，该中心为位于博尔德的科罗拉多大学（CU-Boulder）校园的研究人员提供服务。目前的QqTOF仪器是我们用于分析服务样品的主力，目前被该园区的20多个实验室使用。它主要用于支持合成有机化学和材料开发的研究，包括小分子，天然产物和聚合物的结构和元素分析，以及合成核苷酸和核酸的正负电离模式分析。它还广泛用于蛋白质组学应用，包括氢-氘交换测量以绘制蛋白质-蛋白质和蛋白质-配体界面，以及常规ESI-LC-MS/MS以进行蛋白质组的肽测序以确定蛋白质复合物的亚基组成。目前的QqTOF仪器几乎全天候使用，是中央分析设施的重要组成部分。迫切需要将其替换为QStar Elite QqTOF，以继续支持CU-Boulder的广泛研究需求。 公共卫生相关性：需要资金购买一个新的质谱仪系统，以取代将于2010年因制造商停止维修和服务而退役的现有系统。这是一种用于“称量”分子的关键分析技术，这对生物医学研究至关重要，因为它允许定义分子的化学组成。该仪器将由位于博尔德的科罗拉多大学的20多个实验室共享，他们正在合成新材料或表征蛋白质，目标是了解如何诊断和治疗人类疾病。

DESCRIPTION (provided by applicant): We request funds to purchase an integrated system for liquid chromatography coupled with electrospray ionization mass spectrometry (LC-ESI-MS). The system includes a Applied Biosystems (ABI) QStar Elite quadrupole time-of-flight (QqTOF) mass spectrometer capable of rapid scanning and high accuracy mass measurements, and a Waters ultra-high pressure liquid chromatography system for high resolution analyte separations. The proposed instrument will replace a current ABI QStar Pulsar QqTOF mass spectrometer, which must be retired on July 1, 2010, when ABI terminates all repair and service of the current instrument. The system represents a key instrument at the Central Analytical Facility, which serves investigators across the University of Colorado at Boulder (CU-Boulder) campus. The current QqTOF instrument is our workhorse for service samples analyzed by the facility and is currently used by more than 20 laboratories across this campus. It is most heavily used to support research in synthetic organic chemistry and materials development, including structural and elemental analysis of small molecules, natural products, and polymers, and for negative and positive ionization mode analysis of synthetic nucleotides and nucleic acids. It is also used extensively for proteomics applications, including hydrogen-deuterium exchange measurements to map protein-protein and protein-ligand interfaces, and routine ESI-LC-MS/MS to carry out peptide sequencing of protein digests in order to determine subunit compositions of protein complexes. The current QqTOF instrument is used nearly 24/7, and is an essential component of the Central Analytical Facility. Its replacement with the QStar Elite QqTOF is critically needed to continue supporting a wide range of research needs at CU-Boulder. PUBLIC HEALTH RELEVANCE: Funding is requested to purchase a new mass spectrometry system, to replace an existing system that will be retired in 2010, when the manufacturer discontinues repair and service. This is a key analytical technology for "weighing" molecules, which is essential for biomedical research because it allows the chemical composition of molecules to be defined. The instrument will be shared by more than 20 laboratories at the University of Colorado at Boulder, who are synthesizing new materials or characterizing proteins, with the goal of understanding how to diagnose and treat human diseases.