Leica TCS SP5 Spectral LSCM for the Research Imaging Facility

描述(由申请人提供)：南伊利诺伊大学医学院(SIUSM)请求支持为我们的研究成像核心设施购买新的激光扫描共聚焦显微镜。所要求的徕卡TCS SP5激光扫描共焦显微镜系统将取代1999年由SIUSM购买的奥林巴斯FluoView LSCM，并作为我们唯一的共焦显微镜至今仍在使用。然而，这个装置已经过时了，由于显微镜的年龄，奥林巴斯还不能随时提供支持。获得及时的维修是困难的，也是教师生产力的障碍，显微镜不再满足我们教师的研究需求。徕卡TCS SP5激光扫描共聚焦显微镜系统将支持由NIH资助的具有不同研究兴趣的教职员工的核心，他们的不同资金来源表明。徕卡显微镜将提高数据收集的质量和数量、可用的研究选择、研究生产率和提交同行评议期刊发表的手稿的质量。与我们现有的显微镜相比，新的徕卡系统提供：1)通过减少激发和发射路径中的信号损失来提高灵敏度；2)提高分辨率；3)减少光漂白和光毒性；4)在多个激光谱线的实验设计中具有更大的灵活性；以及5)通过改进的软件应用程序增强分析能力。这种技术的升级将有助于我们更快地了解癌症、传染病、细胞发育以及影响人类健康的细胞和生物分子过程的原因和过程。因此，整体的科学生产力和效率将得到提高。这些好处将使我们的NIH资助的研究人员和其他研究人员能够以其他方式推进他们各自的研究领域，而不是使用SIUSM目前可用的研究工具。

DESCRIPTION (provided by applicant): The Southern Illinois University School of Medicine (SIUSM) requests support to purchase a new laser scanning confocal microscope for our research imaging core facility. The requested Leica TCS SP5 Laser Scanning Confocal Microscope system will replace an Olympus Fluoview LSCM that was purchased in 1999 by SIUSM and remains in use today as our only confocal microscope. However, this unit is outdated and support from Olympus is not readily available due to the age of the microscope. Obtaining timely repairs is difficult and an impediment to faculty productivity and the microscope no longer meets the research needs of our faculty. The Leica TCS SP5 Laser Scanning Confocal Microscope system will support a core of NIH-funded faculty members with diverse research interests as indicated by their varied sources of NIH funding. The Leica microscope will increase the quality and quantity of data collection, available research options, research productivity and quality of manuscripts submitted for publication in peer-reviewed journals. Compared to our existing microscope, the new Leica system offers: 1) improved sensitivity by reducing signal loss in both the excitation and emission pathways; 2) improved resolution; 3) reduced photobleaching and phototoxicity; 4) greater flexibility in experimental design with multiple laser lines; and 5) enhanced analysis capabilities with improved software applications. This upgrade in technology will contribute to more rapid advances in our understanding of the causes and course of cancer, infectious diseases, cell development, and cellular and biomolecular processes affecting human health. Thus, overall scientific productivity and efficiency will be increased. These benefits will allow our NIH-funded and other investigators to advance their respective areas of research in ways not otherwise possible with the research tools currently available to them at SIUSM.