LTQ Orbitrap XL ETD Hybrid FT High Resolution Mass Spectrometer

描述（由申请人提供）：此处建议购买配备高能碰撞解离（HCD）和电子转移解离（ETD）功能的Thermo Scientific LTQ Orbitrap XL ETD混合质谱仪。该仪器将采用Eksigent Nano Ultra 2D液相色谱仪，用于最近由Proteomics Shared Resource购买的肽的复杂混合物的高分辨率纳米流LC分离。该仪器将用于支持由七个主要用户和四个次要用户组成的多样化群体，其中包括在五个部门工作的医生科学家和基础科学家，他们都得到了NIH的广泛资助。这些用户是从范德比尔特大学的160多名主要研究人员中选出的，他们在过去三年中使用了蛋白质组学共享资源，并根据他们对这类仪器所提供的性能水平的更高要求进行选择。LTQ Orbitrap XL ETD将位于Proteomics Shared Resource，这是由范德比尔特大学质谱研究中心运营的三个集中MS核心设施之一。该仪器将由蛋白质组学核心的五名专业人员组成的团队管理，包括仪器操作员和质谱工程师，他们都具有操作和维护Orbitrap的特定经验。业务资金将来自向调查人员征收的用户费，并将采用微软资源中心服务核心多年来一直使用的标准会计和计费程序。一个咨询小组将由三名直接代表用户的高级调查员、一名范德比尔特研究办公室的当然成员和一名独立的高级教员组成。ETD在该仪器上的主要用途是表征蛋白质的翻译后修饰、研究蛋白质-DNA交联和鉴定反应性代谢产物的蛋白质加合物。此外，HCD功能将使定量蛋白质组学研究使用iTRAQ同量异位素标记试剂和其他形式的稳定同位素示踪剂研究，如SILAC标记。 公共卫生相关性：范德比尔特大学的蛋白质组学研究社区得到了NIH的广泛资助。范德比尔特大学的160多个研究小组使用了 自2006年以来的蛋白质组学共享资源。本文提出的Thermo Scientific LTQ Orbitrap XL ETD混合质谱仪将带来重要的新分析能力，用于表征共享资源中现有设备无法提供的改性蛋白质和蛋白质加合物。此外，该仪器将使研究人员能够进行新的实验，例如使用iTRAQ试剂的定量蛋白质组学，这在目前的仪器中是不切实际的。

DESCRIPTION (provided by applicant): Proposed here is the purchase of a Thermo Scientific LTQ Orbitrap XL ETD hybrid mass spectrometer equipped with both high-energy collisional dissociation (HCD) and electron transfer dissociation (ETD) capabilities. The instrument will employ an Eksigent Nano Ultra 2D liquid chromatograph for high resolution nanoflow LC separation of complex mixtures of peptides that was recently purchased by the Proteomics Shared Resource. This instrument will be used to support a diverse group of seven major and four minor users that include physician scientists and basic scientists working in five departments, all of whom are extensively NIH-funded. These users were selected from a base of more than 160 principal investigators at Vanderbilt University who have used the Proteomics Shared Resource over the past three years and selected based on their more demanding need for the level of performance afforded by this class of instrumentation. The LTQ Orbitrap XL ETD will be located in the Proteomics Shared Resource, which is one of three centralized MS core facilities operated by the Vanderbilt University Mass Spectrometry Research Center. The instrument will be managed by a team of five professionals in the proteomics core, including the instrument operator and a mass spectrometry engineer, all of whom have specific experience with operation and maintenance of the Orbitrap. Operational funds will come from user fees assessed to investigators and will employ the standard accounting and billing procedures that have been used by the MSRC service cores for several years. An advisory panel will consist of three senior investigators directly representing the users, an ex officio member of Vanderbilt's Office of Research and an independent senior faculty member. The primary uses for ETD on this instrument will be characterization of posttranslational modifications of proteins, studies of protein-DNA crosslinks and identification of protein adducts of reactive metabolites. In addition, the HCD capabilities will enable quantitative proteomics studies using iTRAQ isobaric tagging reagents and other forms of stable isotopic tracer studies, such as SILAC labeling. PUBLIC HEALTH RELEVANCE: The proteomics research community at Vanderbilt University is extensively funded by NIH. More than 160 investigator groups at Vanderbilt University have used the Proteomics Shared Resource since 2006. The Thermo Scientific LTQ Orbitrap XL ETD hybrid mass spectrometer proposed here will bring important new analytical capabilities for characterization of modified proteins and protein adducts that are unavailable with the current equipment in the Shared Resource. Furthermore, this instrument will enable investigators to undertake new experiments, such as quantitative proteomics using iTRAQ reagents that were hitherto impractical with the current instrumentation.