DEVELOPMENT OF GENOMIC RESOURCES FOR THE PRAIRIE VOLE

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 在本报告所述期间，我们继续创建了代表草原田鼠基因组的6倍BAC文库。使用这个BAC文库，我们分离了包含19个基因的BAC克隆并进行了全序列测定，这些基因目前正在用于 草原田鼠研究社区。 我们使用FISH将其中70个BAC定位到染色体区域。此外，我们还收集了300多只已知血统的草原田鼠的DNA，这些DNA将被用来创建连锁图谱。这些序列已保存在Genbank中。我们已经对256个随机的BAC克隆进行了末端测序，通过对另外3个田鼠的这些基因座进行重新测序，我们在300多个遗传基因座中识别了1000个SNPs。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. During the reporting period, we continued with the creation of the 6x BAC library representing the prairie vole genome. Using this BAC library, we isolated and fully sequenced BAC clones containing 19 genes that are currently being used in the prairie vole research community. We mapped 70 of these BACs to chromosomal regions using FISH. In addition, we collected DNA from over 300 individual prairie voles of known lineage which will be used to create the linkage map. These sequences have been deposited in Genbank. We have end sequenced 256 random BAC clones and by resequencing these loci in 3 additional voles we have identified 1000 SNPs in more than 300 genetic loci.