DNA MICROARRAY AND EXPRESSION CORE

这个子项目是许多利用 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 TNPRC的DNA微阵列和表达核心（微阵列核心）目前提供以下服务：DNA微阵列：1）微阵列实验设计; 2）斑点或双色微阵列实验; 3）微阵列数据分析; 4）小样品扩增; 5）PCR阵列;和6）RNA分离。B。基于siRNA的基因沉默：1）设计和合成对TNPRC群体有用的siRNA的子集; 2）优化和标准化单个siRNA测定。 在2009年期间，核心为各种研究者进行了大约150次微阵列杂交。 总共累积了1.0 TB的原始数据。与去年相比，当时大多数是M。在结核病微阵列方面，今年的大部分工作是宿主微阵列，包括恒河猴微阵列（64）、人类微阵列（12）和哺乳动物微RNA微阵列（20）。所产生的一些数据已经导致出版物（8篇出版物），而更多的数据目前正在分析中。在过去的一年里，核心活动中增加了一项额外的服务：miRNA阵列。这项服务非常受欢迎，已经促成了两项赠款申请的提交，其中一项已经成功，另一项仍在等待。 在未来几年，核心希望扩展到定制微阵列。该核心将设计、制造和生产的前几个阵列将包括恒河猴组织阵列（肠道、大脑）、泛猕猴淋巴芯片和用于监测“无病原体”猕猴群体的多病原体检测芯片。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The DNA Microarray and Expression Core (Microarray Core) at TNPRC currently provides the following services: A. DNA Microarrays: 1) Microarray experimental design; 2) Spotted or 2-color microarray experiments; 3) Microarray Data Analysis; 4) Small Sample Amplification; 5) PCR Arrays; and 6) RNA Isolation. B. siRNA based gene-silencing: 1) Design and synthesis of a subset of siRNA's useful to the TNPRC community; 2) Optimization and standardization of individual siRNA assay. During the year 2009, the core performed approximately 150 microarray hybridizations for various investigators. In all 1.0 Terabytes of raw data was accumulated. In contrast to the prior year when the majority were M. tuberculosis arrays this year the majority of the work were host arrays, including rhesus macaque microarrays (64), human microarrays (12) and mammalian miRNA microarrays (20). Some of the data generated has already lead to publications (8 publications), while much more is currently being analyzed. One additional service was added to the core activities in the last year: miRNA array. This service has been very popular, and has already contributed to the submission of two grant applications, of which one has been successful, while the other remains pending. In the coming years, the core would like to expand into custom microarrays. The first few arrays that will be designed, fabricated and produced by the core will include rhesus macaque tissue arrays (Gut, brain), a pan-macaque lympho-chip and a multi-pathogen detection chip for surveillance of the "pathogen-free" macaque colony.