IN VIVO PROTON MRS REVEALS BRAIN REGION SPECIFIC METABOLIC RESPONSES TO SIV

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 背景：对HIV感染者的体内质子磁共振波谱(1H-MRS)研究表明，不同脑区存在明显的代谢异常，但其原因尚不清楚。用磁共振波谱技术研究了18只SIV感染猕猴额叶皮质、基底节和白质在感染后1个月内的代谢变化。 结果：N-乙酰天门冬氨酸(NAA)、胆碱(Cho)、肌醇(MI)、肌酸(Cr)和谷氨酰胺/谷氨酸(GLX)共振的变化在绝对值和相对肌酸共振值上都是定量的。大多数异常是在病毒血症高峰期，即感染后2周观察到的。此时，仅在额叶皮质观察到反映神经元损伤的NAA和NAA/Cr显著下降。肌酐仅在白质显著升高。各脑区Cho和Cho/Cr的变化相似，在2WPI时升高，4WPI时低于基线水平。MI和MI/Cr水平在所有脑区均升高。 结论：这些数据最好地支持了这样的假设，即不同的大脑区域对艾滋病病毒引起的神经元损伤具有不同的内在易感性。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Background: In vivo proton magnetic resonance spectroscopy (1H-MRS) studies of HIV-infected humans have demonstrated significant metabolic abnormalities that vary by brain region, but the causes are poorly understood. Metabolic changes in the frontal cortex, basal ganglia and white matter in 18 SIV-infected macaques were investigated using MRS during the first month of infection. Results: Changes in the N-acetylaspartate (NAA), choline (Cho), myo-inositol (MI), creatine (Cr) and glutamine/glutamate (Glx) resonances were quantified both in absolute terms and relative to the creatine resonance. Most abnormalities were observed at the time of peak viremia, 2 weeks post infection (wpi). At that time point, significant decreases in NAA and NAA/Cr, reflecting neuronal injury, were observed only in the frontal cortex. Cr was significantly elevated only in the white matter. Changes in Cho and Cho/Cr were similar across the brain regions, increasing at 2 wpi, and falling below baseline levels at 4 wpi. MI and MI/Cr levels were increased across all brain regions. Conclusion: These data best support the hypothesis that different brain regions have variable intrinsic vulnerabilities to neuronal injury caused by the AIDS virus.