UNC Neuroscience Center Research Cores

我们建议北卡罗来纳大学神经科学中心研究核心继续运作，该研究核心由NINDS机构中心核心资助计划资助。这些核心改变了nind资助的北卡罗来纳大学教堂山分校的研究。在之前的资助期内建立了五个科学核心，以支持nind资助的研究人员的基因组学、遗传学和成像。每一个科学核心都取得了显著的成功，这体现在记录的密集和广泛的使用，以及基于使用核心设备和技术支持获得的数据的大量高质量出版物。nind受助者之间以及nind受助者与北卡罗来纳大学教堂山分校其他神经科学家之间的许多合作都受到了使用核心服务的启发。其中三个核心有一个运作良好的充值系统，可以从非NINDS用户那里完全收回费用，并为不能由NINDS中心赠款全额资助的设备升级提供所需资金。

We propose to continue operation of the UNC Neuroscience Center Research Cores funded by the NINDS Institutional Center Core Grant program. These Cores have transformed NINDS-funded research at UNC-Chapel Hill. Five scientific Cores were established in the prior funding period in support of genomics, genetics, and imaging for NINDS-funded investigators. Each of the scientific Cores has been a notable success as manifested by documented intense and broad based usage, and by large numbers of high quality publications based on data obtained using Core equipment and technical support. A number of collaborations among NINDS grantees and between NINDS-grantees and other UNC-Chapel Hill neuroscientists have been inspired by the use of Core services. A well-functioning recharge system is in place for three of the Cores that fully recovers costs from non-NINDS users and provides needed funds for equipment upgrades that cannot be fully funded by this NINDS Center grant. We request continued support of our existing Cores: Core 1: Genomics and Bioinformatics (Affymetrix expression profiling, SNP analysis); Core 2: Expression Localization (in situ hybridization); Core 3: BAG Engineering Technology (vector construction in support of mouse genetics); Core 4: ES Cell Technology (ES cell electroporation and characterization) and Core 5: Confocal and Multiphoton Imaging. We propose to improve our existing Cores with new equipment purchases, implementation of the latest research protocols, provision of new services, and in one case expansion into additional space. In addition, we propose a new Core, Core 6: Assay Development for High Throughput Screening that will develop tools for NINDS-funded investigators to interface with the Translational Proteomics Facility at UNC-Chapel Hill and to access High Throughput Screening Centers established by NINDS and other NIH institutes. The UNC Neuroscience Center Research Cores will be managed via an Administrative Core (Core 7). The UNC-Chapel Hill School of Medicine is fully committed to expansion of research in the area of neurological disease. As evidence of this commitment, the UNC-Chapel Hill School of Medicine now commits a substantial package in support of these Cores and in support of new faculty hires in NINDS priority areas.