Midcareer Investigator Award in Patient-Oriented Research (K24)

描述(申请人提供)：目标。近期和长期目标是：1)建立与肝病相关的翻译、临床和基础研究的领先地位；2)培养下一代从事胃肠道和肝病工作的内科科学家。1 AM致力于加强对医学生、住院医生、研究员和初级教职员工的研究教育培训，促进以患者为导向的研究的发展。研究项目。在这份申请中，1包括了两个以患者为导向的研究项目，这两个项目源于我目前的丙型肝炎中心拨款和一项使用Virahep-C队列的R01拨款。这两个不同的、特征良好的队列被包括在内，以展示将为未来的受训者提供的各种研究。我们研究的中心目标是了解控制丙型肝炎病毒感染恢复的免疫因素，无论是自发的还是治疗诱导的。这里提出的新研究的目的是回答在执行资助项目过程中出现的其他问题。 发展规划。到目前为止，我指导过的大多数人(12人中有11人)都追求学术生涯。来自K24的支持将把我从临床和行政责任中解放出来，让我能够指导更多的实习生，并探索UCHSC杰出科学界的许多合作机会。 研究环境。这项提案中概述的研究将在罗森博士的实验室进行，该实验室占地2707平方英尺，位于UCDS研究综合体2楼。该实验室包括生物危害层流罩、细胞培养二氧化碳孵化器、倒置和立式显微镜、密封式离心机、-70oC和液氮冰柜以及标准的PCR设备。该实验室还配备了FACS Aria、Luminex系统和AID自动Elispot阅读器。邻近的空间可用于分子生物学和蛋白质表达工作。通过Gl部门和科罗拉多州艾滋病研究中心(CFAR)可以获得各种共享设备，包括流式细胞仪和细胞分选仪(Flow Cytometer Core)、高速和超离心机、伽马和贝塔计数器、细胞收割机、荧光显微镜、FPLC和自动ABI 377测序仪。 相关性：全世界约有2亿人患有慢性丙型肝炎病毒感染，很大一部分患者对抗病毒治疗无效。这个K24将增强我指导初级调查人员的能力，并将为这种常见疾病的新免疫治疗方法提供基础。

DESCRIPTION (provided by applicant): Goals. Immediate and long-term objectives are: 1) to be established as a leader in translational, clinical, and basic research related to liver disease; and 2) to train the next generation of physician-scientists working in gastrointestinal and liver disease. 1 am committed to enhancing the educational training of medical students, residents, fellows, and junior faculty members in research and facilitate the growth of patient-oriented research. Research Project. In this application, 1 have included two patient-oriented research projects that stem from my current HCV Center Grant and an R01 grant using the Virahep-C cohort. These two distinct, well-characterized cohorts are included to demonstrate the variety of research that would be available for future trainees. The central goal of our research is to understand the immune factors that govern recovery from HCV infection, either spontaneously or therapy-induced. The purpose of the new research proposed here is to answer additional questions that have arisen in the course of carrying out the funded projects. Development plan. To date, the majority of individuals I have mentored (11 of 12) have pursued academic careers. Support from this K24 will release me from clinical and administrative responsibilities and allow me to mentor of more trainees and explore the many collaborative opportunities within the outstanding scientific community at UCHSC. Research environment. The studies outlined in this proposal will be performed in Dr. Rosen's lab, 2,707 sq ft on the 10th floor of UCDs Research Complex 2. The laboratory contains biohazard laminar flow hoods, cell culture C02 incubators, inverted and upright microscopes, containment centrifuges, -70oC and liquid nitrogen freezers, and standard PCR equipment. The lab also houses a FACS Aria, Luminex system, and an AID automated ELISPOT reader. Adjacent space is available for molecular biology and protein expression work. A wide variety of shared equipment is available through the Gl Division and the Colorado Center for AIDs Research (CFAR), including flow cytometers and cell sorters (Flow Cytometry Core), high speed and ultracentrifuges, gamma and beta counters, cell harvesters, fluorescent microscope, FPLC, and automated ABI 377 sequencer. RELEVANCE: Approximately 200 million throughout the world have chronic HCV infection, and a large proportion of patients fail to respond to antiviral treatment. This K24 will enhance my ability to mentor junior investigators and will provide the groundwork for novel immunotherapeutic approaches of this common disease.