FLOW CYTOMETRY CORE

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 而子项目的主要调查员可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 流式细胞术核心将提供造血干细胞分离、微泡分选和细胞表型分析方面的专业知识。 核心将由马克·杜纳先生领导。 Dooner先生是一位经验丰富的流式细胞仪专家，他将投入80%的时间在这个核心上。 一名待定技术人员将能够为核心提供备份和交叉覆盖。我们已经决定有一个有成就的全职技术人员专门致力于这个COBRE，因为这是合理的复杂性和范围的拟议工作。 该核心采用BD Biosciences的InFlux高速细胞分选仪。该分选机能够进行干细胞分离、单细胞沉积，并安装在生物安全柜内。 这个核心被安置在一个专用的房间里。该设施配备了新的LSRII（BD仪器），配有FACS Diva软件。 研究人员将与核心工作，并准备他们的细胞群进行分类或分析。 核心将常规分离谱系阴性罗丹明lo Hoechst lo（LRH）、LT-HSC、ST-HSC、CLP、CMP、LSK、SLAM和谱系阴性Sca+干/祖细胞群体。 该核心还将对微泡进行分类和表征，并分析种群的谱系特征。 核心将服务于COBRE中的所有项目，执行分离和分析工作。 该核心还致力于开发新的分离技术和增强已知的干细胞和微泡群体。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. The Flow Cytometry Core will provide expertise in hematopoietic stem cell separations, microvesicle sorting, and cell phenotyping analysis. The Core will be headed by Mr. Mark Dooner. Mr. Dooner is an experienced flow cytometrist and will devote 80% time to this Core. A To Be Named Technician will be able to provide backup and cross coverage for the Core. We have decided to have an accomplished full time technician solely dedicated to this COBRE, since this is justified by the complexity and extent of the proposed work. This Core features an InFlux high speed cell sorter from BD Biosciences. This sorter is capable of carrying out stem cell separations, single cell deposition and is housed within a biosafety cabinet. This Core is housed in a dedicated room. The facility is equipped with a new LSRII (BD instruments) with FACS Diva software. Investigators will work with the core and prepare their cell populations for sorting or analysis. The Core will routinely separate Lineage negative Rhodamine lo Hoechst lo (LRH), LT-HSC, ST-HSC, CLP, CMP, LSK, SLAM, and Lineage negative Sca+ stem/progenitor populations. The core will also sort and characterize microvesicles and analyze populations for lineage profiles. The core will serve all projects in this COBRE carrying out both separations and analysis work. The Core also remains dedicated to the development of new separation techniques and the enhancement of known stem cell and microvesicle populations.