TRANSGENIC & GENOTYPING CORE

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 在过去的30年里，转基因小鼠一直是疾病的模型和研究基因功能的工具。尽管它们被广泛使用，但生产这些老鼠所需的技术和设备仍然是专门的。因此，转基因和基因打靶设施通过创造转基因小鼠和提供胚胎服务来支持Cobre研究人员的研究。Core通过原核注射产生转基因小鼠，通过注射囊胚产生嵌合体小鼠，通过电穿孔产生靶向胚胎干细胞，以及提供基因分型和精子冷冻等支持服务。像我们这样的集中式核心设施使每个实验室不需要花费时间和金钱来熟练掌握胚胎处理和转基因小鼠的生产，从而加快研究。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Gene-modified mice have served as models of disease and tools for investigative research into the function of genes for the past 30 years. Despite their widespread use, the techniques and equipment required to produce these mice remain specialized. The Transgenic and Gene-Targeting Facility, therefore, supports the research of COBRE investigators by creating gene-modified mice and providing embryo services. The Core generates transgenic mice by pronuclear injection, chimeric mice by injection of blastocysts and targeted embryonic stem cells by electroporation, as well as providing support services such as genotyping and sperm cryopreservation. A centralized core facility such as ours negates the need for each laboratory to expend the time and money required to become proficient in embryo handling and transgenic mouse production, thereby accelerating research.