Typhoon 9400 Variable Mode Imager for Quantitative Detection of Biomolecules

描述（申请人提供）：宾夕法尼亚大学的研究人员，包括宾夕法尼亚大学医学院、宾夕法尼亚大学兽医学院和费城儿童医院，正在越来越多地参与研究项目，这些项目需要复杂、灵敏和高效的检测技术来研究基因表达、蛋白质生物合成、代谢途径、和分子信号事件。这项技术对于这些机构正在进行的许多NIH资助的研究项目的生产力变得尤为重要。此外，各种迹象表明，在不久的将来，对高度复杂的成像技术的需求将增加。目前，研究人员在这组已经使用了各种传统的检测技术，其中包括CCD相机化学发光，标准磷光成像技术，X射线胶片，线性扫描仪的图像采集和量化设备。单独地，这些技术中的每一种都缺乏当今复杂的细胞信号传导和蛋白质组学方法所需的高通量、足够的灵敏度或足够的空间分辨率。在本申请中，我们建议，常规访问能够检测广泛的报告技术的台式分析仪器将大大促进NIH资助的研究项目目前正在进行的七个主要用户参与本财团的频谱的执行。为了解决这一问题，我们申请购买一种最先进的多模式高通量成像系统，该系统将成熟的存储磷光体放射自显影技术与四色荧光标记和增强的化学发光检测相结合，用于定量分析DNA、RNA和蛋白质样品中的生物分子。拟议的设备，台风9400可变模式成像仪，将被集成到蛋白质和分子生物学核心已经建立在医学实验室的部门设在兽医学院，以促进日益增长的生物分子成像需求的一组合作调查人员从一系列的研究学科在这些学校。

DESCRIPTION (provided by applicant): Researchers at the University of Pennsylvania including the University Of Pennsylvania School Of Medicine, the University Of Pennsylvania School Of Veterinary Medicine and the Children's Hospital of Philadelphia are increasing engaged in research projects that require sophisticated, sensitive, and highly efficient detection techniques for the study gene expression, protein biosynthesis, metabolic pathways, and molecular signaling events. This technology is becoming particularly critical to the productivity of many ongoing NIH funded research projects at these institutions. Furthermore, there is every indication that demand for highly sophisticated imaging techniques will increase in the near future. Currently, investigators in this group have been using a variety of traditional detection techniques which include CCD camera chemiluminescence, standard phosphorimager technology, x-ray film, and linear scanner based image acquisition and quantization devices. Separately, each of these techniques suffers from either a lack of high-throughput, sufficient sensitivity, or adequate spatial resolution required for today's sophisticated cell signaling and proteomics approaches. In this application, we propose that routine access to a bench top analytical instrument capable of detecting a wide range of reporter technologies would greatly facilitate the execution of a spectrum of NIH funded research projects currently being pursued by the seven major users participating in this consortium. To address this, we therefore make application to purchase a state of the art, multimodal, high-throughput imaging system which unites proven storage phosphor autoradiography technology with four color fluorescence labeling and enhanced chemiluminescence detection for the quantitative analysis of biomolecules in DNA, RNA, and protein samples. The proposed equipment, a Typhoon 9400 Variable Mode Imager, will be integrated into the Protein and Molecular Biology Core already established within the Department of Medicine Laboratories housed in the School of Veterinary Medicine for the purpose of facilitating the growing biomolecular imaging needs of a group of collaborative investigators drawn from a range of research disciplines at these Schools.