An 8K camera for the cryoEM at UT Southwestern Medical Center

描述(由申请人提供)：我们建议购买一台B级8k x 8k Tietz F816相机，作为德克萨斯大学西南医学中心高端电子冷冻显微镜(CryoEM；JEOL2200FS FEG)的主要数据采集介质。德克萨斯大学西南分校的低温电子显微镜是达拉斯/福特郡唯一的高端电子显微镜。致力于生物标本成像的沃思区。所要求的相机将取代目前安装在显微镜上的低端相机，将几乎完全消除胶片的使用，并将能够更有效地从各种生物样本中收集数据。它将大大降低使用胶片和其他资源的成本，并将去除显微镜中大多数与胶片有关的污染。以目前可用的摄像头，我们园区的One Scope不能支持本提案中描述的所有项目。拟议的相机将使一个镜头能够有效地用于多个项目，因为它将允许用户更好地利用镜头时间，并在很大程度上消除与胶片相关的停机时间。德克萨斯大学西南校区的十个研究小组将使用所需的摄像头。他们形成了一个强大的用户群体，其项目涵盖了广泛的生物医学研究。这些项目将充分利用我们的CryoEM中所需的数据收集设备。此外，德克萨斯大学西南分校坚定地致力于将低温EM建立为分子和细胞生物学的主要成像技术。它支持购买、维护和服务当前的CryoEM。它现在正在建设一个环境控制更好的新设施，用于再容纳一到两台高端低温EM仪器，这将把低温EM技术的应用扩展到我们园区内许多其他由NIH资助的项目。由于增加了在一个低温EM中支持多个项目的能力，所要求的相机不仅将有利于多个NIH资助的项目，而且还将使德克萨斯大学西南分校实现其成为将低温EM应用于生物医学研究的一流机构的目标。 公共卫生相关性：建议购买一台8k x 8k相机将极大地提高德克萨斯大学西南医学中心电子冷冻显微镜的数据收集效率。它将使冷冻显微镜能够很好地支持NIH资助的多个项目，这些项目面向与健康相关的重要问题。

DESCRIPTION (provided by applicant): We propose to purchase a grade B 8k x 8k Tietz F816 camera, which will serve as a main data- collection medium in the high-end electron cryo-microscope (cryoEM; a JEOL2200FS FEG) at UT Southwestern Medical Center. The cryoEM at UT Southwestern is the only high-end electron microscope in the Dallas / Ft. Worth area that is dedicated to the imaging of biological specimens. The requested camera will replace the low-end cameras currently installed in the microscope, will eliminate the use of film almost completely, and will enable more efficient data- collection from a variety of biological samples. It will greatly reduce the cost of using film and other resources, and will remove most film-related contamination in the microscope. With the currently available cameras, the one scope on our campus is not able to support all projects described in this proposal. The proposed camera will enable the efficient use of one scope for multiple projects because it will allow users to make better use of the scope time and largely eliminate film-related down-time. Ten research groups on the UT Southwestern campus will utilize the requested camera. They form a strong user group with projects that cover a broad spectrum of biomedical research. These projects will fully utilize the requested equipment for data collection in our cryoEM. In addition, UT Southwestern has a strong commitment in establishing cryoEM as a major imaging technique for molecular and cell biology. It has supported the purchase, maintenance, and services of the current cryoEM. It is now constructing a new facility with better-controlled environments for housing one or two more high-end cryoEM instruments, which will extend the application of cryoEM techniques to many other NIH-funded projects on our campus. With the added capability of supporting multiple projects in one cryoEM, the requested camera will not only be beneficial to multiple NIH-funded projects, but also allow UT Southwestern to reach its goal of becoming a premier institution in applying cryoEM to biomedical research. PUBLIC HEALTH RELEVANCE: The proposed purchase of an 8k x 8k camera will greatly improve the data-collection efficiency in the electron cryo-microscope at UT Southwestern Medical Center. It will enable the cryo-microscope to well support multiple NIH-funded projects that are oriented at important health-related questions.