Analytical Glycotechnology

核心单位在项目资助中的资源作用 核心C概述 分析糖技术核心的目的是提供技术专业知识和 分离的唾液酸、N-和O-连接的多糖和GAG的结构表征所需的仪器 来自项目负责人实验室提供的转基因动物的细胞或组织。核心将会 极大地受益于获得现有GlycoTechnology Core中的仪器、试剂和专业知识 加州大学圣迭戈分校糖生物学研究和培训中心(GRTC)的资源。许多技术将成为 用于这些分析(鞘糖脂提取、多糖的分离、唾液酸和唾液酸 衍生分析包括NeuSGc，糖链分析包括末端唾液酸键(a2-3/a2- 6)利用GC-MS、MALDI-TOF MS/MS-MS和2-AB荧光标记的NP-HPLC、GAG 用高效液相色谱和电喷雾质谱(ESI-MS)进行双糖分析)是在上一次资助期间设计的。可得性 高质量的仪器，加上多年的分析糖生物学经验 由Glycotech核心资源的成员代表，使我们能够满足分析 项目负责人提出的挑战，并进行必要的分析，以支持他们的研究。

Function of Core Unit as a Resource to the Program Project Grant Core C Overview The purpose of the Analytical Glycotechnology Core is to provide the technical expertise and instrumentation necessary to structurally characterize sialic acids, N- and O-linked glycans and GAGs isolated from cells or tissues from transgenic animals provided by the Project Leader's laboratories. The core will greatly benefit from access to the instruments, reagents, and expertise in the existing Glycotechnology Core Resource of the UCSD Glycobiology Research and Training Center (GRTC). Many of the techniques to be employed for these analyses (glycosphingolipid extraction, isolation of glycans, sialic acid and sialic acid derivative analysis including NeuSGc, glycosyl linkage analysis including terminal sialic acid linkages (a2-3/a2- 6) using GC-MS, glycan profiling by MALDI-TOF MS/ MS-MS and 2-AB fluorescent labeled NP-HPLC, GAG disaccharide analysis by HPLC and ESI-MS) were devised during the previous funding period. The availability of high quality instrumentation, coupled with the many combined years of analytical glycobiology experience represented by the members of the Glycotechnology Core Resource, allow us to meet the analytical challenges proposed by the Project Leaders and to perform the analyses necessary to support their research.