CORE B: MOLECULAR EXPRESSION AND TRANSGENICS

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 核心B-分子表达和转基因：位于安德森研究大楼104D室(约900平方英尺)新装修的实验室空间。该实验室配备了常规分子和细胞生物学实验的所有主要仪器，包括分光光度计、两台聚合酶链式反应热循环仪、A-80冰柜、两台细胞培养孵化器、两台小型台式离心机和一台电穿孔机等。2008年7月，我们收购了一辆全新的贝顿 Dickinson FACSAria II细胞分选机。Aria有三个激发激光器(真的紫外线、蓝色和红色)和11个荧光探测器，可以比Elite(我们之前使用的，目前仍在使用的)至少快一个数量级的分类速度。我们已经设置了它，并开始使用它来对细胞进行排序。CORE B还有位于安德森111A室的胚胎干细胞培养套间和安德森大楼动物设施内的一个单独的显微注射室。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Core B- Molecular expression and transgenics: is located in the newly renovated lab space in the Anderson research building room 104D (~900 sq.ft). This lab is fully equipped with all major instruments for routine molecular and cellular biology experiments, including a spectrophotometer, two PCR thermal cyclers, a -80 freezer, two cell culture incubators, two mini desktop centrifuges, and an electroporator, etc. Core B has a Backman Coulter Elite cell sorter equipped with an Autoclone for cell sorting. In July 2008, we acquired a brand new Becton Dickinson FACSAria II cell sorter. The Aria has three lasers for excitation (true UV, blue and red) and 11 fluorescence detectors and can sort at least one order of magnitude faster than the Elite (the one that we used before and is still in service). We have set it up and started using for sorting cells. Core B also has ES cell culture suite located in Anderson room 111A and a separate microinjection room inside of the Animal Facility in the Anderson building.