LSU VETERINARY COBRE: MOLECULAR IMMUNOPATHOLOGY CORE

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 路易斯安那州立大学科布雷分子免疫病理学(MIP)核心是由路易斯安那州立大学支持向量机现有的五个中央设施和服务单位组成的联合体，共同提供最先进的服务，以及开发新技术来检测、诊断和分析体外和体内传染病病原体的影响。泽维尔·阿尔瓦雷斯博士(TNPRC)和大卫·斯科拉德博士(NHDP)将参加MIP核心项目。他们的参与确保了已经为非人类灵长类动物和其他动物模型实施的最先进的共聚焦和分子病理学技术转让给LSU-SVM和Cobre-CEIDR的研究人员。MIP联合体由以下现有的支持向量机集中设施组成：1)BIOMMED专注于分子生物学，目前提供先进的重组DNA和cDNA文库构建、实时PCR、DNA测序、BioPlex淋巴因子分析、蛋白质生产和纯化、定制抗体生产等。2)中央病理服务实验室目前提供常规的解剖、临床和分子病理学，后者包括检测病原体和专门细胞的免疫组织化学方法。在若松信子博士(委员会认证病理学家)的指导下，在该服务实验室内成立了一个专门单位。这个单位将是MIP核心的亲密部分，并将作为其他病理服务的基础。3)FACS设施为淋巴因子分析、细胞分类等提供先进的免疫学分析。4)显微镜中心提供光学显微镜、光学显微镜、透射显微镜、电子显微镜、共聚焦显微镜和激光捕获显微镜。MIP核心将开发的新分析方法包括使用原位杂交检测病毒、立克次体和细菌病原体，通过共聚焦和免疫组织化学确定组织免疫概况以确定疾病组织内渗透的免疫细胞的性质等。5)生物统计服务股向路易斯安那州立大学的研究人员提供统计咨询和培训。预计在未来几年，MIP将成为基于LSU-SVM的COBRE-CEIDR的强有力的支持核心，得到LSU-SVM以及其他州和联邦来源的支持。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The Louisiana State University COBRE Molecular Immunopathology (MIP) Core is a consortium arrangement of five existing Centralized Facilities and Service Units of the LSU-SVM working together to provide state-of-the art service, as well as to develop new technologies to detect, diagnose and analyze the effect of infectious disease pathogens in vitro and in vivo. Dr. Xavier Alvarez (TNPRC) and Dr. David Scollard (NHDP) will participate in the MIP Core. Their participation ensures technology transfer of state-of-the art confocal and molecular pathological technologies already implemented for non-human primate and other animal models to researchers at LSU-SVM and the COBRE-CEIDR. The MIP Consortium is composed of the following existing SVM Centralized Facilities: 1) BIOMMED concentrates on molecular biology and currently provides advanced recombinant DNA and cDNA library constructions, real-time PCR, DNA sequencing, BioPlex lymphokine profiling, protein production and purification, custom antibody production etc. 2) The Centralized Pathology Service Laboratory currently provides routine anatomical, clinical and molecular pathology, the later including immuno-histochemical approaches to detect pathogens and specialized cells. A specialized Unit wihin this Service Laboratory has been formed under the direction of Dr. Nobuko Wakamatsu (Board Certified Pathologist). This unit will be an intimate part of the MIP Core and will serve as liason to other pathology services. 3) the FACS Facility provides advanced immunological assays for lymphokine profiling, cell sorting, etc. 4) The Microscopy Center provides, transmission and electron microscopy, confocal microscopy and laser capture microscopy. New assays that will be developed by the MIP Core include the use of in situ hybridization to detect, viral , rickettsial and bacterial pathogens, tissue immunoprofiling to determine via confocal and immuno-histochemistry the nature of infiltrating immune cells within diseased tissues, etc. 5) The Biostatistics Service Unit provides statistical consultation and training to LSU-based researchers. It is expected that MIP will be a strong supporting Core of the LSU-SVM-based COBRE-CEIDR in future years receiving support from LSU-SVM, as well as other state and federal sources.