Metabolic Physiology Core

动物代谢生理学核心旨在满足对具有葡萄糖稳态、胰岛素作用、瘦素作用和/或身体组成改变的啮齿动物模型的表征需求。其主要功能是教授和提供离体和体内方法的咨询，以研究遗传操纵小鼠模型和肥胖/消瘦或胰岛素抵抗/敏感性饮食模型中代谢改变的机制。 核心的具体目标是1）教导和2）为研究人员提供设计，实施和解释体外和体内研究的咨询，以研究胰岛素和调节能量平衡的激素（如瘦素）的作用;葡萄糖稳态;脂质代谢;和身体组成; 3）核心还对由中心成员创建或获得的选定小鼠或大鼠模型进行了有限数量的研究。 核心的服务包括离体方法，如脂肪细胞分离，解剖和培养分离的骨骼肌，以研究葡萄糖转运，胰岛素信号，瘦素信号，葡萄糖和脂肪酸代谢。评估胰岛素作用和葡萄糖稳态的体内方法包括胰岛素耐量试验、葡萄糖耐量试验、响应于胰岛素注射或输注的信号传导测定，以及在钳夹研究期间测量葡萄糖周转、内源性葡萄糖产生和个体组织的葡萄糖摄取。研究促食欲激素和促食欲激素的生物学作用的体内方法包括测量食物摄入、能量消耗和外周组织和下丘脑中的瘦素信号传导 原子核。Core还提供专门的设备，如用于身体成分和骨矿物质密度非侵入性分析的DEXA扫描仪和用于确定脂肪细胞数量和大小分布的Coulter计数器。核心主管芭芭拉B。Kahn，MD，副核心主任，Odile Peroni，PhD和与核心一起工作的研究助理在核心提供的代谢和信号分析方面拥有丰富的经验。他们正在开发新的分析方法来表征综合燃料代谢和能量稳态的其他方面。

The Animal Metabolic Physiology Core is designed to meet the needs for characterization of rodent models that have alterations in glucose homeostasis, insulin action, leptin action and/or body composition. Its main function is to teach and provide consultation on ex vivo and in vivo methods to investigate the mechanisms for metabolic alterations in genetically-manipulated mouse models and in dietary models of obesity/leanness or insulin resistance/sensitivity. The specific objectives of the Core are 1) to teach and 2) to provide consultation to investigators in designing, carrying out and interpreting in vitro and in vivo studies to investigate the action of insulin and hormones that regulate energy balance such as leptin; glucose homeostasis; lipid metabolism; and body composition; 3) The Core also performs a limited number of these studies on selected mouse or rat models created by or obtained by members of the Center. Services of the Core include ex vivo methods such as adipocyte isolation, and dissection and incubation of isolated skeletal muscles to study glucose transport, insulin signaling, leptin signaling, and glucose and fatty acid metabolism. In vivo methods assessing insulin action and glucose homeostasis include insulin tolerance test, glucose tolerance test, signaling assays in response to insulin injection or infusion, and measurement of glucose turnover, endogenous glucose production and glucose uptake by individual tissues during clamp studies. In vivo methods to investigate biological actions of orexigenic and anorexigenic hormones include measurement of food intake, energy expenditure and leptin signaling in peripheral tissues and hypothalamic nuclei. The Core also provides specialized equipment such as a DEXA scanner for non-invasive analysis of body composition and bone mineral density and a Coulter Counter for determining adipocyte number and size distribution. The Core Director, Barbara B. Kahn, MD, the Associate Core Director, Odile Peroni, PhD and the Research Associates who work with the Core have extensive experience with the metabolic and signaling assays offered by the Core. They are developing new assays to characterize additional aspects of integrated fuel metabolism and enerov homeostasis.