STRUCTURE & LIGAND INTERACTION OF GLYCOSYLTRANSFERASES OF THE DOLICOL PATHWAY

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 正在使用资源中开发的NMR方法对Dolicol途径的糖基转移酶进行结构表征。这些是各种糖基化反应所必需的大蛋白质。结构测定对于理解一大类同源蛋白质是重要的。NMR方法，基于单一氨基酸标记策略，残留偶极耦合（RDC）技术，顺磁增强NMR技术，在资源中开发，应用。 目前的目标是Alg 13和Alg 14，它们是将第二个GlcNAc添加到dolicolphosphate的两个亚基蛋白的一部分。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. A structural characterization of glycosyltransferases of the dolicol pathway is being pursued using NMR methods developed in the Resource. These are large proteins essential to various glycosylation reactions. A structure determination is important to understanding a large class of homologous proteins. NMR methods, based on the single amino acid labeling strategy, residual dipolar coupling (RDC) techniques, and paramagnetic enhanced NMR techniques, developed in the resource, are applied. The current targets are, Alg13 and Alg14, parts of a two subunit protein that adds the second GlcNAc to dolicolphosphate.