EPR INVESTIGATIONS OF IRON SIDEROPHORE CHELATES

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 铁载体是一种细菌产物，它结合铁并增加细菌铁的运输速度。一些铁结合化合物也已知在含有动物或人血清的介质中起作用。由于铁封存是已知的感染期间细菌生长的刺激因素，铁载体的合成与细菌的毒力有关。铜绿假单胞菌在限铁条件下产生两种铁载体，它们具有不同的螯合特性，即吡咯啉(PVD)和褐藻胶(PCH)。为了阐明这两个铜绿假单胞菌铁载体中铁铁载体的性质，我们正在进行9.5 GHz低温EPR研究，该研究对铁配体结构的细节特别敏感。该服务项目补充了希望使用中心资源研究铁-蛋白质相互作用的其他中心用户的请求。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Siderophores are bacterial products which bind iron and increase the rate of bacterial iron transport. Some iron-binding compounds are also known to function in media containing animal or human serum. Since iron sequestration is a known stimulus of bacterial growth during infections, siderophore synthesis has been linked to bacterial virulence. Pseudomonas aeruginosa produces two siderophores under iron-limiting conditions with different chelating properties, pyoverdine (PVD) and pyochelin (PCH). In order to elucidate the nature of the iron siderophore ligands in the two P. Aeruginosa siderophores, we are undertaking low temperature 9.5 GHz EPR studies which are particularly sensitive to the details of the iron ligand structure. This service project complements requests from other center users who wish to use center resources for studies of iron-protein interactions.