TRAINING IN MASS SPECTROMETRY AND PROTEOMICS -- MACCOSS LAB 2009-2010

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 Brendan MacLean提供关于Skyline使用的培训 1.在布罗德学院为CPTAC核查工作组操作员会议讲授3个小时的动手教程 2.为Skyline v0.5制作了2 x 25分钟的培训视频 3.核查工作组在WebEx上的定期双月指导更新 4.与核查工作组领导人苏珊·阿巴蒂洛和比尔吉特·席林举行定期WebEx会议 5.对范德比尔特大学、利布勒和塔布实验室进行为期数天的实地考察 6.在苏黎世艾伯赛尔实验室的演示文稿 7.在苏黎世大学小型研讨会上的演讲 8.对卡尔实验室布罗德研究所进行为期数天的实地考察 9.共同教授1.5小时的美国HuPo定量蛋白质组学课程 10.在使用Skyline v0.6中创建了3 x 10-20页的教程 11.在天际支持板上回复了110个支持请求(通过直接电子邮件回复了许多其他请求) 12.与Aebersell实验室的克里斯蒂娜·路德维希进行WebEx会议 13.授课本科生Alana Killeen独立学习专业软件开发 杰西·坎特伯雷提供的一般质谱学分析培训 1.罗布·莫里茨，杰夫·史蒂文斯(ISB)：FAIMS，三重四元运算与数据 2.释义 3.Matt Chambers(Vanderbilt)：为文件读取器代码的单元测试生成数据文件 Shawna Hengel(Med Chem，Goodlett)：ECD实验 5.Scott Shaffer(Med Chem，Goodlett)：离子漏斗和Velos故障排除 6.杰森·伍德(普吉特湾血液中心)：质谱仪和高效液相色谱操作 7.卡特里娜卡爪(斯旺森实验室)：质谱仪操作 Jan Aagaard(Swanson实验室)：质谱仪操作 9.安德鲁·斯特加奇斯(Stamatoyannopoulos实验室)：质谱仪操作 10.肖恩·麦克罗文(Noble)：Dia数据解释 11.米雷拉·安德罗内斯库(诺布尔)：数据转换/解释 丹妮拉·托马泽拉提供的一般质谱学分析培训 1.迈克尔·保尔森：(BChE)Skyline、专栏打包、数据采集 2.小弗朗西斯科·诺西蒂(牙骨质工程)样品制备：胰酶消化、数据采集、MSDaPl 3.凌旺(Baker Lab)：数据采集 4.Judit Marsillach(Furlong Lab/OP项目)：胰酶消化、MCx多肽样品清理、柱填充 5.安德鲁·斯特加奇斯(Stamatoyannopoulos实验室)：天际线、MS方法设置、SRM数据分析 6.Jason Wood(普吉特湾血液中心)：天际线，LTQ SRM数据采集，SRM数据分析 7.Tomas Vaisar(Heinecke Lab)：使用Skyline的预定SRM 谢长廷提供的一般质谱学分析培训 1.Katrina Cavw(Swanson Lab)：填充毛细管柱和使用安捷伦1100 HPLC 2.刀夫刀(Rabinovitch Lab)：填充毛细管柱，用Waters Nanquity和Thermo LTQ-FT仪器进行样品分析，并进行数据分析。 彼得·施特劳布(Vanderbilt)：与Bullseye进行数据分析。 4.Lea Starita(Fields Lab)：使用Bullseye和毛细管柱填充进行数据分析。 由Gennifer Merrihew提供的培训 1.MSDaPl/MacCoss实验室管道帮助-Evvie Vincoe，Lea Starita，Jan Aagard，Melody Rynerson，Katrina Cavw，Renee George，Angela Poole 2.Orbitrap运行，样本准备讨论-Evvie Vincoo，Lea Starita 3.GelFree-Lea Starita，Andrew Stergacits，Chuck Witkowski，Jeremy Norris 4.LTQ分析帮助，专栏制作帮助-Melody Rynerson，Katrina Cavw，Renee George，Jan Aagard，Angela Poole，Lea Starita 5.古代蛋白质样本Prep-Ben Novak，Tom Kaye，Matthew Fitzgibbon，Pavel Pezner，Martin McIntosh 6.关于同位素标签的讨论--Soledad Undraraga 7.TAP标记的FOXO细胞系--Matt Kaeberlein，Lara Shamieh

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Training Provided by Brendan MacLean on the Use of Skyline 1. Taught 3 hours of hands-on tutorials for CPTAC Verification Working Group Operators meeting at Broad Institute 2. Created 2 x 25 minute training videos for Skyline v0.5 3. Regular bimonthly instructional updates over WebEx for Verification Working Group 4. Regular WebEx sessions with Susan Abbatiello and Birgit Schilling, leaders of Verification Working Group 5. Multi-day site visit at Vanderbilt University, Liebler and Tabb labs 6. Presentation to Aebersold Lab in Zurich 7. Presentation at U. Zurich mini-symposium 8. Multi-day site visit at Broad Institute, Carr lab 9. Co-taught 1.5 hours of US HUPO quantitative proteomics course 10. Created 3 x 10-20 page tutorials in using Skyline v0.6 11. Answered 110 support requests on Skyline support board (many others through direct email) 12. WebEx sessions with Christina Ludwig of Aebersold Lab 13. Taught undergraduate Alana Killeen professional software development for independent study Training Provided by Jesse Canterbury on General Mass Spectrometry Analysis 1. Rob Moritz, Jeff Stevens (ISB): FAIMS, triple quad operation & data 2. interpretation 3. Matt Chambers (Vanderbilt): generating data files for unit tests of file reader code 4. Shawna Hengel (Med Chem, Goodlett): ECD experiments 5. Scott Shaffer (Med Chem, Goodlett): ion funnel & Velos troubleshooting 6. Jason Wooden (Puget Sound Blood Center): mass spec & HPLC operation 7. Katrina Claw (Swanson lab): mass spec operation 8. Jan Aagaard (Swanson lab): mass spec operation 9. Andrew Stergachis (Stamatoyannopoulos lab): mass spec operation 10. Sean McIlwain (Noble): DIA data interpretation 11. Mirela Andronescu (Noble): data conversion/interpretation Training Provided by Daniela Tomazela on General Mass Spectrometry Analysis 1. Michael Paulsen: (BChE) Skyline, Column Packing, Data Acquisition 2. Francisco Nociti Jr. (Cementum project) Sample Preparation: Tryptic Digestion, Data Acquisition, MSDaPl 3. Ling Wang (Baker Lab): Data Acquisition 4. Judit Marsillach (Furlong lab / OP Project): Tryptic Digestion, MCX Peptide Sample Cleanup, Column Packing 5. Andrew Stergachis (Stamatoyannopoulos lab): Skyline, MS Method setup, SRM data Analysis 6. Jason Wooden (Puget Sound Blood Center): Skyline, LTQ SRM data acquisition, SRM data analysis 7. Tomas Vaisar (Heinecke Lab): Scheduled SRM using Skyline Training Provided by Ed Hsieh on General Mass Spectrometry Analysis 1. Katrina Claw (Swanson Lab): packing capillary columns and use of Agilent 1100 HPLCs 2. Dao-Fu Dao (Rabinovitch Lab): packing capillary columns, with sample analysis on Waters nanoAcquity and Thermo LTQ-FT instruments and with data analysis. 3. Peter Straub (Vanderbilt): in data analysis with Bullseye. 4. Lea Starita (Fields Lab): in data analysis with Bullseye and capillary column packing. Training Provided by Gennifer Merrihew 1. MSDaPl / MacCoss lab pipeline help - Evvie Vincow, Lea Starita, Jan Aagard, Melody Rynerson, Katrina Claw, Renee George, Angela Poole 2. Orbitrap runs, sample prep discussions - Evvie Vincow, Lea Starita 3. GelFree - Lea Starita, Andrew Stergachis, Chuck Witkowski, Jeremy Norris 4. LTQ analysis help, column making help - Melody Rynerson, Katrina Claw, Renee George, Jan Aagard, Angela Poole, Lea Starita 5. Ancient Protein Sample Prep - Ben Novak, Tom Kaye, Matthew Fitzgibbon, Pavel Pezner, Martin McIntosh 6. Isotope labeling discussions - Soledad Undurraga 7. TAP-tagged FOXO cell lines - Matt Kaeberlein, Lara Shamieh