COBRE: U OF KANSAS MEDICAL CTR: MOLECULAR BIOLOGY CORE

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 分子生物学核心将为所有Cobre科学家提供技术支持、最先进的设备和必要的分子生物学试剂，以便以经济高效的方式进行需要分子生物学方法的实验。CORE提供和维护的设施包括一台用于DNA测序的ABI Prism 3100遗传分析仪、两台实时PCR机、一台Biomek FX分析工作站(液体分配器)、五台用于聚合酶链式反应的热循环仪，以及其他常用的分子生物学设备，如具有密度分析能力的成像系统、细菌培养箱和超速离心机。核心负责维护和培训研究人员使用这些设施。此外，该中心还维护探针、引子、抗体、细胞系、小鼠组织和质粒的库存数据库，供科布雷研究人员共享。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. The Molecular Biology Core will provide all COBRE scientists with technical support, state-of-the-art equipment, and essential molecular biology reagents, so that experiments that require a molecular biology approach can be carried out in a cost-effective way. The facilities provided and maintained by the Core include an ABI Prism 3100 Genetic Analyzer for DNA sequencing, two real-time PCR machines, a Biomek FX Assay Work Station (liquid dispenser), five thermocyclers for polymerase chain reaction, and other commonly used molecular biology equipment, such as an imaging system with the capability for densitometry analysis, bacteria incubators, and ultracentrifuges. The Core is responsible for maintaining and training researchers to use these facilities. In addition, the Core maintains inventory databases for probes, primers, antibodies, cell lines, mouse tissues, and plasmids, which can be shared by COBRE investigators.