Flow Cytometry Core

流式细胞术是细胞表型分析中应用最广泛和不可缺少的技术之一 以及用于分离特定的细胞群体。流式细胞术核心资源由POl支持 以具有成本效益的方式为该计划的调查人员提供更多的工具， 分析流式细胞术，在某些情况下必须保持在适当的生物安全容器中 条件核心有三个具体目标：1）提供和维护必要的研究 流式细胞术研究所需的基础设施，以及适当的生物安全防护措施; 2） 在涉及流动的既定技术的执行和解释方面提供技术援助 3）引入新技术并更新现有技术，以保持尖端能力。的 由PO支持的核心设施完全负责维护一台分析流式细胞仪， 有助于一个高速细胞分选机的维护和操作。通过此核心访问 在生物安全水平（BSL）-2和BSL-3条件下的流式细胞仪分析和分选技术， 在过去的5年里，在由该POL赠款资助的研究中发挥了重要作用，并将继续这样做， 下一个融资期。仪器的高成本和对熟练人员的需求使得 建立流式细胞术的核心设施是管理这些疾病的唯一实用方法。 资源此外，这一核心设施提供了唯一的可能性，为政治调查员做流 在该机构内的BSL-3环境中进行细胞计数，因此是一些研究的关键设施 正在进行或计划中的项目。核心还提供了一个环境， 哪些生物安全控制程序可以得到严格监督和执行，从而确保 在与潜在危险的传染性病原体的工作中使用流式细胞术技术的人员。的 核心的分析能力使项目研究人员能够识别和表征细胞群 在与分枝杆菌发病机制和宿主免疫应答相关的广泛研究中具有重要意义。 相关性（参见说明）： 该提案要求支持一个核心设施，该设施将提供复杂的设备， P01计划中的研究人员分析细胞群体的各种分子的表达。的 将得到支持的方法对于将进行的许多类型的实验至关重要 以实现设计和构建更好的疫苗和药物来预防结核病的总体目标。

Flow cytometry is one of the most widely used and indispensable techniques for analysis of cell phenotypes and for isolation of specific cell populations. The Flow Cytometry Core resources supported by the POl provide the Program's investigators with increased access in a cost effective way to instruments for analytical flow cytometry, which in some cases must be maintained in appropriate biosafety containment condifions. The core has three specific aims: 1) Provide and maintain the necessary research infrastructure needed for flow cytometry studies, with appropriate biosafety containment safeguards; 2) Provide technical assistance in the performance and interpretafion of established techniques involving flow cytometry; 3) Introduce new and update existing technologies to maintain cutting edge capabilifies. The core facility supported by the POl has full responsibility for maintaining one analytical flow cytometer, and contributes to the maintenance and operation of one high speed cell sorter. Access through this core to FACS analysis and sorting techniques under Biosafety Level (BSL)-2 and BSL-3 condifions has played an essential role in research funded by this POl grant during the past 5 years, and will continue to do so into the next funding period. The high cost ofthe instrumentafion and the need for skilled personnel makes the establishment of a core facility for flow cytometry the only pracfical approach to management of these resources. In addition, this core facility provides the only possibility for the POl investigators to do flow cytometry in a BSL-3 environment within the institution, and thus is a crucial facility for some of the studies that are ongoing or planned within the context of the program. The core also provides an environment in which biosafety containment procedures can be stricfiy overseen and enforced, thus ensuring the safety of personnel using flow cytometry techniques in their work with potentially dangerous infectious agents. The analytical capabilifies of the core allow the program investigators to identify and characterize cell populations of interest in a wide range of studies related to mycobacterial pathogenesis and the host immune response. RELEVANCE (See instructions): This proposal requests support for a core facility that will provide sophisficated equipment to allow investigators in the POl Program to analyze cell populations for their expression of various molecules. The methods that will be supported are of crucial importance to many types of experiments that will be performed to address the overall goal of designing and constructing better vaccines and drugs to prevent tuberculosis.