Using Arabidopsis to uncover interactions between phytohormone signaling pathways

项目总结-植物激素信号通路的相互作用 应聘者：候选人的长期职业目标是成为一名学术领域的首席研究员 背景，研究参与植物激素反应的相互作用网络。在培训期间， 她将学习生化研究方法，以补充她的研究生应用农业和博士后- 分子遗传学博士研究。 培训环境：莱斯大学以其高质量提供理想的培训环境 研究和私密的环境。Bonnie Bartel博士，这个项目的导师，是拟南芥领域的领导者 研究。此外，她是一位积极的导师，将大部分时间投入到她的研究和她的 博士后和学生。 研究：该项目的长期目标是加强对信令网络的了解 连接植物激素生长素、脱落酸(ABA)和乙烯。双特异性蛋白 磷酸酶，IBR5，这一提议的主题，是生长素、ABA和 乙烯信号转导网络。几种相互关联的方法将使用IBR5来获得更大的收益 了解这些植物激素之间的关系。首先，IBR5相互作用的蛋白质将是 已确定(目标1)；这些可能包括IBR5磷酸酶的底物和调节剂。另外， 将分析第二位点的ibr5修饰物(目标2)，以确定是否对生长素、ABA和 乙烯可以分离，或者如果它们以不可分割的方式相互连接。瑕疵的鉴定 这些修饰物中的基因(目标3)将允许从分子上阐明它们之间的相互作用。 植物激素途径。这些研究将有助于理解哪些信令组件 在这三条路径之间共享且不共享的路径，并将标识 植物激素信号网络。除了扩展我们的基础知识，详细了解 在这三种植物激素中，与生长反应、耐受胁迫和果实成熟有关的激素可能 为最终改良具有农业或药用价值的植物提供见解。例如, 确定如何在不改变生长特性(生长素)的情况下提高抗逆性(ABA感知) 感知)或成熟(乙烯感知)可能是植物适应高胁迫区域(即区域)的关键 低降雨量或盐碱地)。

PROJECT SUMMARY - INTERACTION OF PHYTOHORMONE SIGNALING PATHWAYS Candidate: The candidate's long-term career goal is to become a principal investigator in an academic setting, studying the interaction networks involved in phytohormone response. During the training period, she will learn biochemical research approaches to complement her graduate applied agricultural and post- doctoral molecular genetic research. Training Environment: Rice University provides an ideal training environment because of its quality research and intimate setting. Dr. Bonnie Bartel, the mentor for this project, is a leader in Arabidopsis research. Further, she is an active mentor who devotes the majority of her time to her research and her postdocs and students. Research: The long-term goal of this project is to enhance understanding of the signaling network that connects the phytohormones auxin, abscisic acid (ABA), and ethylene. The dual-specificity protein phosphatase, IBR5, the subject of this proposal, is a novel point of interaction between auxin, ABA, and ethylene signal transduction networks. Several interconnected approaches will use IBR5 to gain greater understanding of the relationship among these phytohormones. Firstly, IBR5-interacting proteins will be identified (Aim 1); these may include substrates and regulators of the IBR5 phosphatase. Additionally, second-site ibr5 modifiers will be analyzed (Aim 2) to determine whether sensitivity to auxin, ABA, and ethylene can be separated, or if they are interconnected in inseparable ways. Identification of defective genes in these modifiers (Aim 3) will allow molecular elucidation of interactions between these phytohormone pathways. These studies will contribute to understanding of which signaling components are shared among these three pathways and which are not shared, and will identify additional nodes in the phytohormone signaling network. In addition to expanding our basic knowledge, a detailed understanding of these three phytohormones, involved in growth responses, stress tolerance, and fruit ripening, may provide insight for the eventual improvement of plants of agricultural or medicinal importance. For instance, determining how to increase stress tolerance (ABA perception) without altering growth characteristics (auxin perception) or ripening (ethylene perception) could be key to adapting plants to high stress areas (i.e., areas of low rainfall or saline soils).