Musculoskeletal Structure and Strength

组织结构和强度是评估与以下相关的动物模型时最相关的性质： 肌肉骨骼损伤、疾病和修复。治疗效果是否是由基因 操作，代谢挑战，模拟损伤，手术修复或其他干预，净效应必须 根据它们是否导致了更多或更少的组织，该组织是否具有正常的 形态，以及组织或骨骼结构是否具有增加或降低的机械性能。 我们有必要的设备和证明用户的专业知识，以评估结构和 来自动物模型的一系列肌肉骨骼组织和结构的机械性能。 研究基地的调查人员与研究中心的工作人员进行了富有成效的合作， 这一核心，但由于缺乏支持收购的机制， 专家用户分析数据和培训非专家用户。本肌肉骨骼的目的 结构和强度核心（核心B）是提供一种机制，以增加对现有资源的访问， 基于X射线的密度测定和成像以及机械测试，从而实现新的相互作用， 加强华盛顿大学肌肉骨骼研究人员之间现有的互动。我们将 维护协议和设备，以促进质量控制，提供技术支持和培训，以及 对动物模型（小鼠至 犬）由核心中心研究基地的研究人员生成。目标1：X射线成像; 可用的方式包括放射照相术、双能X射线吸收测定法（DXA）、外周定量 计算机断层扫描（pQCT）和微计算机断层扫描（microCT）。每一个都可以用于 体内或体内成像。目的2：力学测试;可用的方式包括全骨弯曲和 脱矿骨、肌腱和肌腱-骨插入的压缩、小梁压痕、拉伸试验 部位拉伸试验;肌力测量。通过提供这些核心服务，我们将增加研究 在我们的校园建立肌肉骨骼研究人员的生产力，并促进非肌肉骨骼 希望为肌肉骨骼生物学和医学研究带来新视角的研究人员。

Tissue structure and strength are the most relevant properties when assessing animal models related to musculoskeletal injury, disease and repair. Whether the treatment effects are created by genetic manipulation, metabolic challenge, simulated injury, surgical repair or other intervention, the net effects must be judged based on whether they have resulted in more or less tissue, whether that tissue has normal morphology, and whether the tissue or skeletal structure has increased or decreased mechanical properties. We have the necessary equipment and the demonstrated user expertise to assess the structural and mechanical properties of a range of musculoskeletal tissue and structures derived from animal models. There have been productive collaborations between investigators in the Research Base and the personnel of this Core, but these have been limited in number by the lack of a mechanism to support the acquisition and analysis of data by expert users and the training of non-expert users. The objective of this Musculoskeletal Structure and Strength Core (Core B) is to provide a mechanism to increase access to existing resources for x-ray based densitometry and imaging, and mechanical testing, and to thereby enable new interactions and enhance existing interactions between musculoskeletal researchers at Washington University. We will maintain protocols and equipment to promote quality control, provide technical support and training, and perform the following services on musculoskeletal structures and tissues from animal models (mouse to canine) generated by investigators in the Core Center Research Base. Aim 1: X-ray based imaging; available modalities include radiography, dual-energy x-ray absorptiometry (DXA), peripheral quantitative computed tomography (pQCT) and micro-computed tomography (microCT). Each of these is available for ex vivo or in vivo imaging. Aim 2: Mechanical testing; available modalities include whole-bone bending and compression, trabecular indentation, tensile testing of demineralized bone, tendon and tendon-bone insertion site tensile testing; muscle force measurement. By offering these Core services we will increase research productivity of established musculoskeletal investigators on our campus and facilitate non-musculoskeletal investigators wishing to bring new perspectives to studies in musculoskeletal biology and medicine.