CUMC: IMAGING CORE

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 该成像设备是一种新的多用户核心，与结构生物学的科学焦点密切相关。核心是基于获得LSM 510 METANLO共焦扫描系统，使用三种激光(Argon 458/477/488/514 nm，Green Hene 543 nm，Red Hene 633 nm)。共焦建立在蔡司AxioPlan2IE MOT电动立式显微镜上，目标如下：计划Neo 40x OIL 1.3数值孔径(n.a.)具有差分干涉对比度(DIC)；计划63x 1.4 n.a。Achroplan 40x 0.8 n.a.水DIC，用于活细胞工作；C-Apochromat 40x 1.2 n.a.DIC，用于荧光相关光谱；计划Apo 10x；计划Neo 20x 0.75 n.a.迪克。核心的初始资金是通过NSF EPSCoR赠款提供的。Core可以在单光子或多光子模式下与大多数荧光团一起成像，分辨率接近理论极限(222 Nm)。Meta多通道系统能够通过线性分离来分离具有密集发射峰的荧光团。现有的实验类型远远超过了传统的高分辨率共聚焦成像。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. This imaging facility is a new multiuser core that is closely related to the scientific focus in structural biology. The core is based upon the acquisition of LSM 510 METANLO confocal scanning system with three lasers (Argon 458/477/488/514 nm, Green HeNe 543 nm, Red HeNe 633 nm. The confocal is built on a Zeiss AxioPlan 2IE MOT motorized upright microscope, with the following objectives: Plan Neo 40x oil 1.3 numerical aperture (n.a.) with differential interference contrast (DIC); Plan 63x 1.4 n.a. DIC; Achroplan 40x 0.8 n.a. water DIC, for live cell work; C-Apochromat 40x 1.2 n.a. DIC ,for fluorescence correlation spectroscopy; Plan Apo 10x; Plan Neo 20x 0.75 n.a. DIC. Initial funding for the core was through an NSF EPSCoR grant. The Core can image with most fluorophores, in either single-photon or multi-photon modes, with resolution approaching the theoretical limit (222 nm) The META multi-channel system enables separation of fluorophores with closely-spaced emission peaks by linear un-mixing. The types of experiments available far surpass those of conventional high resolution confocal imaging.