UNC Neuroscience Center Research Cores

我们建议继续由NINDS机构中心核心资助计划资助的神经科学中心研究核心的运作。这些核心已经改变了NINDS资助的研究在北卡罗来纳大学教堂山。在前一个资助期内建立了五个科学核心，以支持NINDS资助的研究人员的基因组学，遗传学和成像。每一个科学核心都取得了显著的成功，这体现在有记录的密集和广泛的使用，以及大量基于使用核心设备和技术支持获得的数据的高质量出版物。NINDS受资助者之间以及NINDS受资助者与其他UNC教堂山神经科学家之间的许多合作都受到了核心服务的启发。一个运作良好的充电系统是到位的三个核心，完全收回成本，从非NINDS用户，并提供所需的资金，设备升级，不能完全由这个NINDS中心赠款。 我们要求继续支持我们现有的核心：核心1：基因组学和生物信息学（Affyphine表达谱，SNP分析）;核心2：表达定位（原位杂交）;核心3：BAG工程技术（支持小鼠遗传学的载体构建）;核心4：ES细胞技术（ES细胞电穿孔和表征）和核心5：共聚焦和多光子成像。我们建议通过购买新设备，实施最新的研究协议，提供新的服务，并在一个情况下扩展到额外的空间来改善我们现有的核心。此外，我们提出了一个新的核心，核心6：高通量筛选的检测开发，将为NINDS资助的研究人员开发工具，与北卡罗来纳大学教堂山分校的翻译蛋白质组学设施接口，并访问NINDS和其他NIH研究所建立的高通量筛选中心。神经科学中心研究核心将通过管理核心（核心7）进行管理。 北卡罗来纳大学教堂山医学院完全致力于扩大神经系统疾病领域的研究。作为这一承诺的证据，北卡罗来纳大学教堂山医学院现在承诺提供大量资金支持这些核心，并支持NINDS优先领域的新教师招聘。

We propose to continue operation of the UNC Neuroscience Center Research Cores funded by the NINDS Institutional Center Core Grant program. These Cores have transformed NINDS-funded research at UNC-Chapel Hill. Five scientific Cores were established in the prior funding period in support of genomics, genetics, and imaging for NINDS-funded investigators. Each of the scientific Cores has been a notable success as manifested by documented intense and broad based usage, and by large numbers of high quality publications based on data obtained using Core equipment and technical support. A number of collaborations among NINDS grantees and between NINDS-grantees and other UNC-Chapel Hill neuroscientists have been inspired by the use of Core services. A well-functioning recharge system is in place for three of the Cores that fully recovers costs from non-NINDS users and provides needed funds for equipment upgrades that cannot be fully funded by this NINDS Center grant. We request continued support of our existing Cores: Core 1: Genomics and Bioinformatics (Affymetrix expression profiling, SNP analysis); Core 2: Expression Localization (in situ hybridization); Core 3: BAG Engineering Technology (vector construction in support of mouse genetics); Core 4: ES Cell Technology (ES cell electroporation and characterization) and Core 5: Confocal and Multiphoton Imaging. We propose to improve our existing Cores with new equipment purchases, implementation of the latest research protocols, provision of new services, and in one case expansion into additional space. In addition, we propose a new Core, Core 6: Assay Development for High Throughput Screening that will develop tools for NINDS-funded investigators to interface with the Translational Proteomics Facility at UNC-Chapel Hill and to access High Throughput Screening Centers established by NINDS and other NIH institutes. The UNC Neuroscience Center Research Cores will be managed via an Administrative Core (Core 7). The UNC-Chapel Hill School of Medicine is fully committed to expansion of research in the area of neurological disease. As evidence of this commitment, the UNC-Chapel Hill School of Medicine now commits a substantial package in support of these Cores and in support of new faculty hires in NINDS priority areas.