Portable Lab-on-a-chip Flow Cytometer: Prototype and Application Development

描述（由申请人提供）：便携式芯片实验室流式细胞仪：原型和应用开发NanoSort研究及相关其他项目信息7.项目概述这里提出的芯片实验室NanoSort设备推进了第一阶段Beta原型项目的成就。该装置将通过改善分选流式细胞术的可及性并降低分选流式细胞术的成本和复杂性而适合于商业成功。此外，它将扩展该设备可以服务的应用，包括用于诊断，预后和个性化医疗的转化和临床即时流式细胞术应用。在完成第一阶段计划的关键里程碑后，如可量产的注塑芯片、先进的信号检测和处理以及关键性能指标，我们建议扩展与同类最佳设备竞争的能力。我们这个项目的目标是创建一个设备（NanoSort-1或NS-1），将有一个单一的光电倍增管（PMT），使用低剪切应力压电（PZT）驱动器的4路分选6色检测改进的性能参数，和一个强大的数字信号处理算法（颜色-空间-时间（CoST）的实现，允许每秒10,000个事件的检测和排序。除了这些创新之外，我们还将使用当前的良好制造工艺（cGMP）来构建设备，以确保该设备将获得UL和CE认证，并可扩展到每年10，000台。我们将通过咨询UCSD和器械制造商（Becton-Dickinson和Life Technologies）的知名研究人员来验证该器械。 公共卫生相关性：便携式芯片实验室流式细胞仪：原型和应用开发NanoSort研究及相关其他项目信息8.生物医学研究中的重要技术包括细胞或颗粒的计数（流式细胞术）和分离（细胞分选）基于其固有属性，目前由非常大（~ 800磅）和昂贵（~ 50万美元）的机器进行，需要专门的空间和工作人员进行操作。我们发明了三种技术并获得专利，这些技术使分选流式细胞仪的开发成本和尺寸仅为传统仪器的一小部分，使用微流体通道细胞，压电致动器分选细胞，以及一种称为颜色-空间-时间编码的技术，用单个检测器检测多种颜色（而不是传统仪器中的每种颜色一个检测器）。成本和复杂性的降低使得任何研究人员都可以使用这种新的纳米分选设备，推进生物医学发现。

DESCRIPTION (provided by applicant): Portable Lab-on-a-chip Flow Cytometer: Prototype and Application Development NanoSort RESEARCH & RELATED Other Project Information 7. PROJECT SUMMARY The lab-on-a-chip NanoSort device proposed here advances the achievements of the Phase I Beta Prototype project. This device will be appropriate for commercial success by improving access to and reducing the cost and complexity of sorting flow cytometry. Furthermore, it will expand the applications that this device can serve, including translational and clinical point-of-care flow cytometry applications for diagnostics, prognosis, and personalized medicine. Having completed key milestones in the Phase I program, such as mas- producible injection molded chips, advanced signal detection and processing and key performance metrics, we propose to extend the capabilities to compete with best-in-class devices. Our Aims for this project are to create a device (NanoSort-1 or NS-1) that will have improved performance parameters of 6-color detection with a single photomultiplier tube (PMT), 4-way sorting using low shear stress piezoelectric (PZT) actuators, and a robust digital signal processing algorithm (Color-Space-Time (CoST) implementation that allows 10,000 events per second detection and sorting. In addition to these innovations, we will construct the device using current good manufacturing processes (cGMP) to assure that this device will be UL and CE certified and scalable to 10,000 units per year. We will validate this device with consultation from established researchers at UCSD and device manufactures (Becton-Dickinson and Life Technologies). PUBLIC HEALTH RELEVANCE: Portable Lab-on-a-chip Flow Cytometer: Prototype and Application Development NanoSort RESEARCH & RELATED Other Project Information 8. PROJECT NARRATIVE Important techniques in biomedical research include the counting (flow cytometry) and separation (cell sorting) of cells or particles based on their innate properties, currently performed by very large (~800lbs) and expensive (~$500,000) machines that require dedicated space and staff for their operation. We have invented and patented three techniques that enable the development of a sorting flow cytometer at a fraction of the cost and size of conventional instruments, using microfluidics to channel cells, piezoelectric actuators to sort cells, and a technique called Color-Space-Time coding to detect several colors with a single detector (as opposed to one detector per color in conventional instruments). Reductions in costs and complexity make this new nanosorting device accessible to any researcher, advancing biomedical discovery.