Molecular Profiling and Pathology

核心服务计划说明：分子图谱和病理学(MPP)核心的主要目的 是为波兰的调查人员提供一个集中和协调的资源，用于分析 人肺癌标本和动物模型组织的形态和高通量分子研究 方法(组织病理学、免疫组织化学、原位杂交、基因组和蛋白质组分析)。 该POL中的MPP核心职能被归类为四个逻辑上相互关联的目标或活动。1. 采购用于验证研究的人肺癌组织。核心将作为集中的 使用在几个MKSCC IRBs批准下获得的人肺癌样本进行验证研究的资源 协议。用于再活组织检查方案(治疗开始后的非治疗性再活组织检查或再活组织检查 一旦由于假定的获得性耐药而进展)，戈尔也将作为主要组织 存储库。2.病理回顾与分析。MPP核心将为组织学和细胞学提供专家 对POL中使用的所有人类肺癌标本进行评估和分类，包括基于形态的 检测(IHC、GISH、FISH)。MPP还将提供对#年肺肿瘤的组织病理学专家审查 小鼠模型以及异种移植的人类肿瘤。3.人类肺癌组织的基因分型 验证性研究。MPP戈尔已经建立并正在进一步发展预期的基因分型工作 提供对其人类肺肿瘤资源的扩展突变注释，这对于合理、良好设计至关重要 验证研究与研究项目中产生的特定线索具有生物学相关性。4. 使用完全注释的人类肺癌组织进行验证研究的协调。所描述的活动 在AIMS 1-3中，将提供丰富的严格分类的人类肺癌组织集合，这些组织被注释为关键 肺癌基因。验证由单个研究项目产生的发现所需的研究 在人类肺癌组织中，将由核心人员或由核心人员协调执行。健壮的平台是 为DNA、RNA和蛋白质分析物建立，具体验证分析由 个人研究项目要回答的问题。

Description of Core Service Plan: The primary purpose ofthe Molecular Profiling and Pathology (MPP) Core is to provide the investigators in this POl with a centralized and coordinated resource for the analysis of human lung cancer samples and animal model tissues using morphologic and high throughput molecular methods (histopathology, immunohistochemistry, in situ hybridization, genomic and proteomic analyses). The MPP Core functions within this POl are grouped into four logically connected Aims or activities. 1. Procurement of human lung cancer tissues for validation studies. The Core will serve as the centralized resource for validation studies using human lung cancer samples procured under several MKSCC IRBapproved protocols. For rebiopsy protocols (non-therapeutic rebiopsy after treatment initiation or rebiopsy upon progression due to presumed acquired resistance), the Gore will also serve as the main tissue repository. 2. Pathology review & analysis. The MPP core will provide expert histologic and cytologic evaluation and classification of all human lung cancer specimens used in the POl, including morphologybased assays (IHC, GISH, FISH). The MPP will also provide expert histopathologic review of lung tumors in mouse models as well as xenografted human tumors. 3. Genotyping of human lung cancer tissues for validation studies. The MPP Gore has established and is further developing prospective genotyping efforts to provide extended mutational annotation of its human lung tumor resources that is critical for rational, welldesigned validation studies biologically relevant to specific leads arising from the Research Projects. 4. Coordination of validation studies using fully annotated human lung cancer tissues. The activities described in Aims 1-3 will provide a rich collection of rigorously classified human lung cancer tissues annotated for key lung cancer genes. Studies needed to validate the discoveries generated by the individual research projects in human lung cancer tissues will be performed in or coordinated by Core personnel. Robust platforms are established for DNA, RNA and protein analytes, with the specific validation analyses being determined by the questions to be answered by the individual Research Projects.